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I5654

Sigma-Aldrich

IgG3, Kappa from human myeloma plasma

purified immunoglobulin, >90% (microfluidic capillary gel electrophoresis)

Synonim(y):

Human IgG3-κ

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About This Item

Numer MDL:
Kod UNSPSC:
12352203
NACRES:
NA.46

pochodzenie biologiczne

human

Poziom jakości

białko sprzężone

unconjugated

forma przeciwciała

purified immunoglobulin

Próba

>90% (microfluidic capillary gel electrophoresis)

Warunki transportu

dry ice

temp. przechowywania

−20°C

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Opis ogólny

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG3 is the has a unique extended hinge region conferring greater flexibility
Human myeloma IgG3, κ is purified from human plasma by fractionation, ion-exchange, and affinity chromatography procedures.

Zastosowanie

IgG3, kappa from human myeloma plasma has been used to determine the trisulfide levels in commercial therapeutic IgG1 antibodies and in human myeloma IgG1, IgG2, IgG3 and IgG4 antibodies.
The purified IgG3, κ may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. Human myeloma IgG3 was for in flow cytometry in CHO cells.

Postać fizyczna

Solution in 20 mM tris buffered saline, pH 8.0.

Komentarz do analizy

The purity and identity are determined by immunoelectrophoresis, indirect ELISA and SDS-PAGE.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Characterization of trisulfide modification in antibodies
Gu S, et al.
Analytical Biochemistry, 400(1), 89-98 (2010)
Rangaiah Shashidharamurthy et al.
Journal of immunology (Baltimore, Md. : 1950), 183(12), 8216-8224 (2009-12-17)
CD32A, the major phagocytic FcgammaR in humans, exhibits a polymorphism in the ligand binding domain. Individuals homozygous for the R allelic form of CD32A (CD32A(R) allele) are more susceptible to bacterial infections and autoimmune diseases as compared with H allelic
David R Martinez et al.
Cell, 178(1), 190-201 (2019-06-18)
The placental transfer of maternal IgG is critical for infant protection against infectious pathogens. However, factors that modulate the placental transfer of IgG remain largely undefined. HIV-infected women have impaired placental IgG transfer, presenting a unique "disruption model" to define
Masayuki Hirano et al.
Nature immunology, 8(7), 762-771 (2007-06-15)
Because functional analysis of Fc receptors (FcRs) relies heavily on mouse models, the identification of another Fcgamma receptor is particularly noteworthy. We demonstrate that FcgammaRIV, identified here as the mouse ortholog of primate FcgammaRIII, required association of the FcR gamma-chain
S Hashira et al.
Pediatrics international : official journal of the Japan Pediatric Society, 42(4), 337-342 (2000-09-15)
Maternal immunoglobulin G (IgG), transferred across the placenta to the fetus during intrauterine life, is an important component of the neonatal immunological defence mechanisms against infection. There is controversy with respect to differences in placental transfer of the different IgG

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