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Merck

I5260

Sigma-Aldrich

Anti-Human IgG (Fab specific) antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonim(y):

Fab-Specific Antibody

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About This Item

Numer MDL:
Kod UNSPSC:
12352203
NACRES:
NA.46

pochodzenie biologiczne

goat

Poziom jakości

białko sprzężone

unconjugated

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

secondary antibodies

klon

polyclonal

Postać

buffered aqueous solution

metody

indirect ELISA: 1:50,000
quantitative precipitin assay: 2.0 mg/mL

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

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Opis ogólny

Anti-Human IgG (Fab specific) antiserum is produced in goat using purified human IgG Fab fragment as the immunogen. Affinity isolated antibody is obtained from goat anti-human IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. IgG is a larger molecule with a molecular weight of 150 kilo daltons (kDa). IgG has two light chains (L) and two heavy chains (H). The light chain can be either lambda (λ) or kappa (κ) chain. Functionally, immunoglobulins have constant region and variable region. Light chain has one variable region in the N-terminal VL and followed by one constant domain CL. The light chain is approximately about 25 kDa. The heavy chain has one variable region in the N-terminal and followed by three constant domains. In between the first (C1H) and second constant (C2H) domain in the spacer hinge region which connects both the heavy chains. Each heavy chain is about 55 kDa.

Specyficzność

Goat anti-human IgG (Fab specific) antibody reacts specifically with Fab fragment of human IgG and shows no reactivity for Fc fragment of human IgG. This product has no interspecies cross reactivity for mouse and rat IgG.

Immunogen

Purified human IgG Fab fragment.

Zastosowanie

Anti-Human IgG (Fab specific) antibody produced in goat is effective as a second antibody reagent in immunoassay procedures. It may be used:
  • as starting material for conjugates using enzymes or fluorescent dyes
  • in enzyme linked immunosorbent assay
  • as a cross-linking antibody in internalisation of 125I-IgG by streptolysin-O-permeabilised cells
  • as capture antibody in enzyme-linked immunosorbent assay (ELISA) for detection and semiquantitation of Fab
  • in electrophoresis and immunoelectrophoresis

Działania biochem./fizjol.

Enzymatic cleavage of IgG yields antigen-binding (Fab) and effector activating (Fc) fragments. IgG treatment with papain produces two Fab fragments and one Fc fragment. IgG treatment with pepsin produces (Fab′)2 fragment alone. IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus they play a crucial role in facilitating cytological immune responses. Anti-human IgG (Fab specific) antibody can be used in Ouchterlony double diffusion. Due to lack of interspecies cross reactivity to mouse or rat ascites fluids, this antibody is also ideal for screening human monoclonal antibodies produced by hybridoma cells grown in vivo.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Li Du et al.
Radiation oncology (London, England), 5, 70-70 (2010-08-14)
Overexpression of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is commonly occurred in cancers and causes radioresistance and poor prognosis. In present study, the single-chain variable antibody fragments (scFv) targeting DNA-PKcs was developed for the application of radiosensitization in vitro and
Richard J Kraus et al.
Journal of virology, 94(18) (2020-07-10)
We previously reported that the cellular transcription factor hypoxia-inducible factor 1α (HIF-1α) binds a hypoxia response element (HRE) located within the promoter of Epstein-Barr virus's (EBV's) latent-lytic switch BZLF1 gene, Zp, inducing viral reactivation. In this study, EBV-infected cell lines
Human recombinant antibodies against Plasmodium falciparum merozoite surface protein 3 cloned from peripheral blood leukocytes of individuals with immunity to malaria demonstrate antiparasitic properties
Lundquist R, et al.
Infection and Immunity, 74(6), 3222-3231 (2006)
Stabilized, long-term expression of heterodimeric proteins from tricistronic mRNA
Mielke C, et al.
Gene, 254(1-2), 1-8 (2000)
Edurne Rujas et al.
Cell reports, 32(7), 108037-108037 (2020-08-20)
The contribution of membrane interfacial interactions to recognition of membrane-embedded antigens by antibodies is currently unclear. This report demonstrates the optimization of this type of antibodies via chemical modification of regions near the membrane but not directly involved in the

Produkty

Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.

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