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Merck

H8651

Sigma-Aldrich

HEPES sodium salt

BioXtra, ≥99.0% (titration)

Synonim(y):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt

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About This Item

Wzór empiryczny (zapis Hilla):
C8H17N2NaO4S
Numer CAS:
Masa cząsteczkowa:
260.29
Numer WE:
Numer MDL:
Kod UNSPSC:
12161700
Identyfikator substancji w PubChem:
NACRES:
NA.25

linia produktu

BioXtra

Próba

≥99.0% (titration)

Postać

powder

zanieczyszczenia

≤0.005% Phosphorus (P)
≤0.1% Insoluble matter

pH

10.0-12.0 (1 M in H2O)

przydatny zakres pH

6.8-8.2

pKa (25°C)

7.5

rozpuszczalność

H2O: 1 M, clear, colorless

ślady anionów

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

ślady kationów

Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Pb: ≤0.001%
Zn: ≤0.0005%

absorpcja

≤0.2 at 260 in H2O at 1 M
≤0.2 at 280 in H2O at 1 M

Zastosowanie

diagnostic assay manufacturing

ciąg SMILES

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

Klucz InChI

RDZTWEVXRGYCFV-UHFFFAOYSA-M

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Opis ogólny

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of 6.8 to 8.2.

Zastosowanie

HEPES sodium salt solution is suitable for cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during the phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Inne uwagi

Easily compare specifications for HEPES sodium salt products with the HEPES sodium salt specification table.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


Certyfikaty analizy (CoA)

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C Estrada et al.
The Biochemical journal, 326 ( Pt 2), 369-376 (1997-09-18)
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I J Chopra et al.
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We have devised a practical, sensitive, and reliable assay for measurement of free T3 concentration in serum. The assay employs a convenient and disposable plastic equilibrium dialysis cell and a buffer that resembles the in vivo biochemical environment (Nelson JC
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An in situ prepared Zn(2+) complex of triazole linked imino-thiophenyl conjugate of calix[4]arene, [ZnL], was demonstrated to be highly fluorescent in HEPES buffer solution. [ZnL] has been used as a chemo-sensing ensemble for the recognition of phosphates in general and
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