H5041
Cystatin C human
recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
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About This Item
Polecane produkty
pochodzenie biologiczne
human
Poziom jakości
rekombinowane
expressed in HEK 293 cells
Próba
≥95% (SDS-PAGE)
Postać
lyophilized powder
siła działania
≤5 μM IC50
jakość
endotoxin tested
masa cząsteczkowa
dimer 12-13 kDa (non-glycosylated)
opakowanie
pkg of 10 μg
warunki przechowywania
avoid repeated freeze/thaw cycles
metody
cell culture | mammalian: suitable
zanieczyszczenia
≤1 EU/mg
numer dostępu UniProt
temp. przechowywania
−20°C
informacje o genach
human ... CYTC(1471)
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Opis ogólny
HumanKine human Cystatin C is expressed as a non-glycosylated monomer in human HEK 293 cells. Cystatin C belongs to the cystatin superfamily. Two isoforms (pI = 7.8, 9.2) of native Cystatin C are found in human urine differentiated by the elimination of small basic peptides or amino acids from the N-terminal end of the protein. This protein is coded by a housekeeping gene, CST3 located on human chromosome 20p11.21 and is generated by nucleated cells.
Zastosowanie
Cystatin C human has been used as a standard in western blot assay. It has also been used to study its effect on the endopeptidase activity of activated Trichobilharzia regenti isoform of cathepsin B1 peptidase (TrCB1).
Działania biochem./fizjol.
Cystatin C is an inhibitor of cysteine proteases including cathepsin B which has been identified as the most important β-amyloid-degrading enzyme. Measurement of cystatin C in serum is replacing creatinine as an indicator of kidney function (glomerular filtration rate, GFR). Studies show its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer′s disease. Cystatin C inhibits transforming growth factor β signaling in normal and cancer cells.
Postać fizyczna
Lyophilized from a 0.2 μm filtered solution of 1×PBS
Komentarz do analizy
The inhibitory function of cystatin c on papain′s protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC50 value was measured at 5 to 20 μg/mL (0.3 to 1.5 μM) with a range of 1.56 μg/mL to 50 μg/mL cystatin C in presence of 0.55 μM papain and 0.44 μM L-BAPA.
Informacje prawne
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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Journal of proteome research, 9(12), 6101-6111 (2010-10-05)
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Hana Dvořáková et al.
Frontiers in cellular and infection microbiology, 10, 66-66 (2020-03-17)
Schistosomula (the post-infective stages) of the neurotropic schistosome Trichobilharzia regenti possess multiple isoforms of cathepsin B1 peptidase (TrCB1.1-TrCB1.6) with involvement in nutrient digestion. The comparison of substrate preferences of TrCB1.1 and TrCB1.4 showed that TrCB1.4 had a very narrow substrate
Hyun Jung Chung et al.
PloS one, 10(7), e0133417-e0133417 (2015-07-18)
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