H2663

Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal

clone HDAC2-62, purified from hybridoma cell culture

• Numer MDL: MFCD02096544
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 200
• białko sprzężone: unconjugated
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: HDAC2-62, monoclonal
• Postać: buffered aqueous solution
• masa cząsteczkowa: antigen ~55 kDa
• reaktywność gatunkowa: human, chicken, bovine, canine, rat, mouse
• opakowanie: antibody small pack of 25 μL
• metody: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.25-0.5 μg/mL using total cell extracts from NIH3T3 cells
• izotyp: IgG2b
• numer dostępu UniProt: Q92769
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... HDAC2(3066) ; mouse ... Hdac2(15182) ; rat ... Hdac2(84577)

Opis

Opis ogólny

Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the HDAC2-62 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C-terminal region of human histone deacetylase 2 (amino acid residues with N-terminal added cysteine) conjugated to maleimide-activated KLH. Histone deacetylase 2 (HDAC2) is the catalytic subunits of different multiprotein regulatory complexes.

Specyficzność

Monoclonal Anti-Histone Deacetylase 2 (HDAC2) recognizes human, bovine, dog, rat, mouse, and chicken HDAC2 (approx. 55 kDa). The antibody epitope resides within amino acids 471-488 of human HDAC2.

Zastosowanie

Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal has been used in:

• co-immunoprecipitation
• chromatin immunoprecipitation (ChIP)
• western blot
• immunohistochemistry
• enzyme linked immunosorbent assays (ELISA)

Działania biochem./fizjol.

Histone Deacetylases including HDAC′s 1-10 facilitate transcriptional repression and nucleosome condensation. Abnormal recruitment of HDACs by various oncoproteins may occur in certain neoplastic diseases. Inhibition of HDAC2 activity by valproic acid induces proteasomal degradation of HDAC2.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)