Przejdź do zawartości
Merck
Wszystkie zdjęcia(1)

Key Documents

View All Documentation

G7663

Sigma-Aldrich

Gelatin blocking buffer

for Western blotting, powder blend

Synonim(y):

blocking buffer

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wszystkie zdjęcia(1)

About This Item

Kod UNSPSC:
41105300
NACRES:
NA.25

klasa czystości

for molecular biology

Poziom jakości

100

Postać

powder blend

temp. przechowywania

room temp

Opis ogólny

Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).

Zastosowanie

Suitable for use as a non-specific blocking agent for Western blots.

Rekonstytucja

Dissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.
This page may contain text that has been machine translated.

produkt powiązany

Numer produktu
Opis
Cennik

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

A Fortin et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 72(5-6), 239-243 (1994-05-01)
The major advantages of the horseradish peroxidase chemiluminescence (HRP-CL) immunodetection method in Western blot analysis are its high sensitivity, nonradioactive detection, economy of the primary antibody, and speed of detecting the signal. However, we observed a strong and reproducible signal
K A Brogden et al.
Infection and immunity, 67(8), 4256-4259 (1999-07-23)
Affinity-purified rabbit polyclonal (PAB96-1) and mouse monoclonal (1G9-1C2) antibodies to synthetic H-DDDDDDD-OH, an antimicrobial anionic peptide (AP) originally isolated from ovine pulmonary surfactant, were prepared and used to assess the concentrations of AP-like molecules in human respiratory tract samples. In
A sensitive cell-based assay for the detection of residual infectious West Nile virus.
Koldijk, M.H., et al.
Vaccine, 25, 39-39 (2007)
Raul Bettencourt et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 152(2), 278-289 (2008-12-02)
The interaction between microorganisms and host defense mechanisms is a decisive factor for the survival of marine bivalves. They rely on cell-mediated and humoral reactions to overcome the pathogens that naturally occur in the marine environment. In order to understand
Iram Liaqat et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 43(3), 969-980 (2012-07-01)
Enterotoxigenic Escherichia coli (ETEC) strains are leading causes of childhood diarrhea in developing countries. Adhesion is the first step in pathogenesis of ETEC infections and ETEC pili designated colonization factor antigens (CFAs) are believed to be important in the biofim
Wyświetl wszystkie powiązane dokumenty

Protokoły

Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting

In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.

Protokoły Northern i Southern Blot

Poznaj podstawy blottingu Northern i Southern, wraz z protokołami i aplikacjami do przenoszenia makrocząsteczek na nośniki membranowe.

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej