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Dokumenty

G4509

Glycerokinase from Escherichia coli

Name: Glycerokinase from <I>Escherichia coli</I>
Brand: SIGMA

lyophilized powder, 40-100 units/mg protein

Synonim(y):

ATP:glycerol 3-phosphotransferase, Glycerol Kinase

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About This Item

Numer CAS:

9030-66-4

Numer EC enzymu:

2.7.1.30 (BRENDA, IUBMB)

Numer WE:

232-862-0

Numer MDL:

MFCD00131205

Kod UNSPSC:

12352204

NACRES:

NA.54

pochodzenie biologiczne

Escherichia coli

Postać

lyophilized powder

aktywność właściwa

40-100 units/mg protein

skład

Protein, ≥50% biuret

obecność zanieczyszczeń

Triose-phosphate isomerase, hexokinase, myokinase, glycerol dehydrogenase and NADH oxidase ≤0.05%

temp. przechowywania

−20°C

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Zastosowanie

Glycerol kinase (glpK) was used to study the effects of pain controlling neuropeptides on human fat cell lipolysis.

Działania biochem./fizjol.

Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.

Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. <<<20,21>>> It is also subject to feedback regulation by fructose-1,6-bisphosphate.<<<22>>>

Definicja jednostki

One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH.

Postać fizyczna

Partially purified lyophilized powder, balance is primarily salts and EDTA.

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Piktogramy

GHS08

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H334

Zwroty wskazujące środki ostrożności

P261 - P284 - P501

Klasyfikacja zagrożeń

Resp. Sens. 1

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

Certyfikaty analizy (CoA)

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Sigma-Aldrich

L9518

Lipase from Pseudomonas sp.

Mechanisms involved in the regulation of free fatty acid release from isolated human fat cells by acylation-stimulating protein and insulin.

V Van Harmelen et al.

The Journal of biological chemistry, 274(26), 18243-18251 (1999-06-22)

The effects of acylation-stimulating protein (ASP) and insulin on free fatty acid (FFA) release from isolated human fat cells and the signal transduction pathways to induce these effects were studied. ASP and insulin inhibited basal and norepinephrine-induced FFA release by

Compartmentalized system with membrane-bound glycerol kinase. Activity and product distribution versus asymmetrical substrate supply.

A Girard et al.

The Biochemical journal, 274 ( Pt 3), 819-824 (1991-03-15)

An artificial-membrane-bound glycerokinase chosen as a membrane-bound two-substrate-enzyme model has been used to separate two unequal compartments of a specially designed diffusion cell. An interesting feature is the asymmetry of compartments and the existence of a diffusion layer adjacent to

Effects of pain controlling neuropeptides on human fat cell lipolysis.

V van Harmelen et al.

International journal of obesity (2005), 34(8), 1333-1340 (2010-03-17)

Neuropeptides NPFF and NPSF are involved in pain control, acting through the G-protein coupled receptors (GPR)74 (high affinity for NPFF) and GPR147 (equal affinity for NPFF and NPSF). GPR74 also inhibits catecholamine-induced adipocyte lipolysis and regulates fat mass in humans.

The whey fermentation product malleable protein matrix decreases TAG concentrations in patients with the metabolic syndrome: a randomised placebo-controlled trial.

Ioanna Gouni-Berthold et al.

The British journal of nutrition, 107(11), 1694-1706 (2011-10-15)

Animal and human studies suggest that a malleable protein matrix (MPM) from whey decreases plasma lipid concentrations and may positively influence other components of the metabolic syndrome such as glucose metabolism and blood pressure (BP). The primary objective of this

Capacity of bioregulators of stem and progenitor cells to strongly affect liver redox-dependent processes.

Daria V Cherkashina et al.

Rejuvenation research, 14(6), 661-667 (2011-10-20)

Abstract Effects of stem and progenitor cells or their compounds on recipient cells are investigated intensively today. In spite of this, their ability to interact with native cells and the final targets affected by them, particularly biochemical parameters that characterize

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Protokoły

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

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