F6258
Anti-Rat IgG (whole molecule)–FITC antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Synonim(y):
Goat Anti-Rat IgG (whole molecule)–Fluorescein isothiocyanate
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Polecane produkty
pochodzenie biologiczne
goat
Poziom jakości
białko sprzężone
FITC conjugate
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
secondary antibodies
klon
polyclonal
Postać
buffered aqueous solution
metody
direct immunofluorescence: 1:32
temp. przechowywania
2-8°C
docelowa modyfikacja potranslacyjna
unmodified
Powiązane kategorie
Opis ogólny
An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Rat IgG (whole molecule)-FITC antibody is specific for all rat IgG subclasses. Goat anti-rat IgG is purified by affinity isolation and then conjugated to FITC.
IgG is the main antibody type found in plasma and extracellular fluid and is expressed on B cell membrane. It regulates several functions such as complement activation and phagocytosis and hence plays a pivotal role in facilitating cytological immune responses. Anti-rat IgG (whole molecule)-FITC antibody can be used as a secondary antibody in indirect immunofluorescence. Goat anti- rat IgG (whole molecule)-FITC antibody reacts specifically with all rat immunoglobulins.
Immunogen
Purified rat IgG
Purified rat IgG.
Zastosowanie
Anti-Rat IgG (whole molecule)−FITC antibody has been used in immunofluorescence measurement of antibody-treated cells and staining of monolayers.
Anti-Rat IgG (whole molecule)-FITC antibody may be used for immunofluorescent labeling of rat spleen cells. For immunofluorescence of rat cartilage tissue sections, a concentration of 5-10 μg/ml was used. The range of dilutions of anti-rat IgG used for immunofluorescence is between 1:10 and 1:250 in various studies.. The antibody was also used in flow cytometry applications.
Anti-rat IgG (whole molecule)-FITC antibody (diluted 1:20 in PBS) can be used as a secondary antibody in immunolabelling. It can also be used in flow cytometry.
Postać fizyczna
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This page may contain text that has been machine translated.
Nie możesz znaleźć właściwego produktu?
Wypróbuj nasz Narzędzie selektora produktów.
Kod klasy składowania
10 - Combustible liquids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Klienci oglądali również te produkty
Sim K Singhrao et al.
Microscopy research and technique, 72(7), 501-506 (2009-03-21)
Immunofluorescence labeling on postembedded tissue in resin is a formidable task. Although resin components and stabilizers are a source of additional strong native fluorescence that overlaps with absorption and emission spectra of commonly used green fluorophores, the unfixed tissue is
T A Yednock et al.
The Journal of cell biology, 104(3), 725-731 (1987-03-01)
Blood-borne lymphocytes extravasate in large numbers within peripheral lymph nodes (PN) and other secondary lymphoid organs. It has been proposed that the initiation of extravasation is based upon a family of cell adhesion molecules (homing receptors) that mediate lymphocyte attachment
The Laboratory Rat (2005)
Aged HCT-8 cell monolayers support Cryptosporidium parvum infection
Sifuentes L Y, et al.
Applied and Environmental Microbiology, 73(23), 7548-7551 (2007)
Molecular Genetics of Immunoglobulin (1987)
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej