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E7908
p3XFLAG-CMV™-14 Expression Vector
shuttle vector for transient or stable intracellular expression of C-terminal 3xFLAG
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About This Item
Kod UNSPSC:
12352200
Polecane produkty
znacznik
3X FLAG tagged
klasa czystości
for molecular biology
Postać
buffered aqueous solution
Warunki transportu
dry ice
temp. przechowywania
−20°C
Opis ogólny
The p3XFLAG-CMV™-14 Expression Vector is a 6.3 kb derivative of pCMV51 used to establish expression of transient or stable intracellular C-terminal 3XFLAG™ fusion proteins in mammalian cells. The vector encodes three adjacent FLAG® epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) downstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. Efficiency of replication and genomic integration is optimal when using an SV40 T antigen-expressing host.
The p3XFLAG-CMV-7-BAP Control Plasmid is a 6.2 kb derivative of pCMV5 used for transient intracellular expression of N-terminal 3XFLAG bacterial alkaline phosphatase fusion protein in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein.
Vector Maps and Sequences
The p3XFLAG-CMV-7-BAP Control Plasmid is a 6.2 kb derivative of pCMV5 used for transient intracellular expression of N-terminal 3XFLAG bacterial alkaline phosphatase fusion protein in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein.
Vector Maps and Sequences
Komponenty
- p3XFLAG-CMV™-14 Expression Vector 20 μg (E4901) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
- p3XFLAG-CMV™-7-BAP Control Plasmid 20 μg (C7472) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
Zasada
The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG®-fusion constructs
Inne uwagi
Browse additional application references in our FLAG® Literature portal.
Informacje prawne
This product is covered by the following patents owned by Sigma-Aldrich Co. LLC: US6,379,903, US7,094,548, JP4405125,EP1220933, CA2386471 and AU774216.
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
p3xFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
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10 - Combustible liquids
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The present study compared properties of wild-type and mutated mouse and human myocilin (Myoc) proteins as a prerequisite for development of a mouse model of glaucoma. cDNA encoding full-length mouse Myoc was cloned into the p3XFLAG-CMV-14 vector. Tyr423His and Ile463Ser
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A splice variant of cyclooxygenase-1 (COX-1), COX-1b (previously termed as COX-3), has been identified in canine tissues as an acetaminophen-sensitive isoform, but the sequence of COX-1b mRNA and the encoded protein are not known in rats. We cloned and sequenced
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XPC protein is a critical DNA damage recognition factor in nucleotide excision repair for which genetic deficiency confers a predisposition to cancer. In this study, we show that XPC has a function that is independent of its canonical function in
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