DUO92021

Duolink^® In Situ PLA^® Probe Anti-Human MINUS

Affinity purified Donkey anti-Human IgG (H+L)

• Synonim(y): Odczynnik do testu interakcji białko-białko, Odczynnik do testu ligacji zbliżeniowej in situ
• Kod UNSPSC: 41105331
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: donkey (Polyclonal)
• forma przeciwciała: affinity purified immunoglobulin (secondary antibody)
• linia produktu: Duolink^®
• reaktywność gatunkowa: human
• metody: immunofluorescence: suitable; proximity ligation assay: suitable
• przydatność: suitable for brightfield; suitable for fluorescence
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C

Opis

Zastosowanie

Duolink^® proximity ligation assay(PLA^®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

This product can be applied to both the Duolink^® In Situ Fluorescence Protocol and the Duolink^® In Situ Brightfield Protocol depending on the detection reagents used.

Visit our Duolink^® PLA Resource Center for information on how to run a Duolink^® experiment, applications, troubleshooting and more.

To perform a complete Duolink^® PLA^® in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink^® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment. HRP is also available for brightfield detection.

Specificity

PLA probe anti-Human reacts with whole molecule human IgG and the light chains of other human immunoglobulins. The PLA Probe Anti-Human Minus has minimal cross-reactivity with bovine, chicken, goat, guinea pig, Syrian hamster, horse, mouse, rabbit, rat, and sheep serum proteins. A PLUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information.

Application Note

Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink^® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink^® projects

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Cechy i korzyści

• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Single molecule sensitivity due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Quicker and simpler than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

Komponenty

This product is comprised of the following:

• 5x PLA Probe Anti-Human MINUS - Donkey anti-human secondary antibody conjugated to oligonucleotide MINUS
• 1x Blocking Solution - Reagent for blocking of the sample
• 1x Antibody Diluent - For dilution of PLA probes and primary antibodies

See datasheet for more information.

Informacje prawne

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany

PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07,GHS09
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H317 - H411
• Zwroty wskazujące środki ostrożności: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Klasyfikacja zagrożeń: Aquatic Chronic 2 - Skin Sens. 1
• Kod klasy składowania: 10 - Combustible liquids