Key Documents
DUO82047
Duolink® In Situ Wash Buffer, Brightfield
Synonim(y):
in situ Proximity Ligation Assay Kit, Protein Protein Interaction Assay reagent
About This Item
Polecane produkty
Materiały
packing (powdered buffer pouches)
Poziom jakości
linia produktu
Duolink®
metody
proximity ligation assay: suitable
przydatność
suitable for brightfield
temp. przechowywania
20-25°C
Zastosowanie
Follow the Duolink® In Situ Brightfield Protocol to use this product.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using HRP for brightfield detection.
Duolink® In Situ Wash Buffer, Brightfield is used to wash specimen slides during Duolink® In Situ Brightfield procedures. See datasheet for more details.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
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Cechy i korzyści
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Informacje prawne
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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Produkty
Support information including tips and tricks, frequently asked questions, and basic troubleshooting.
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Things to consider for preparation, setup and execution of the Duolink® assay protocol
Protokoły
This protocol describes the use of Duolink® PLA reagents for the brightfield detection, visualization, and quantification of individual proteins, protein modifications, and protein interactions in tissue and cell samples.
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
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