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D4904
pBR322 Plasmid DNA from E. coli RRI
buffered aqueous solution
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About This Item
Numer CAS:
Numer WE:
Numer MDL:
Kod UNSPSC:
12352200
eCl@ss:
32160414
NACRES:
NA.51
Polecane produkty
rekombinowane
expressed in E. coli
Poziom jakości
klasa czystości
Molecular Biology
for molecular biology
Formularz
buffered aqueous solution
masa cząsteczkowa
2.9 MDa
4363 bp
Pochodzenie replikacji
BR322
Wybór
ampicillin
Warunki transportu
dry ice
temp. przechowywania
−20°C
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Opis ogólny
Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in E. coli. This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives. Plasmid pBR322 confers resistance to ampicillin and tetracycline. The plasmid sequence has been published.
The plasmid has unique restriction sites within the gene for ampicillin resistance (Pst I, Pvu I, and Sca I), within the gene for tetracycline resistance (BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, and Xma III), and elsewhere (Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, and Tth111 I).
The plasmid has unique restriction sites within the gene for ampicillin resistance (Pst I, Pvu I, and Sca I), within the gene for tetracycline resistance (BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, and Xma III), and elsewhere (Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, and Tth111 I).
Specyficzność
Unique Sites: Within the gene for ampicillin resistance: Pst I, Pvu I, Sca I; Within the gene for tetracycline resistance: BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, Xma III; other unique sites: Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, Tth111 I.
Zastosowanie
Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in Escherichia coli. This plasmid and derivatives have been used for a number of purposes including cloning, selection and expression of recombinant molecules, construction of shuttle vectors and vectors for nucleotide sequencing, studies of elements involved in gene expression, as plasmid DNA standards, and as a model system for studies on prokaryotic plasmid replication.
pBR322 Plasmid DNA from E. coli RRI has been used to study about ferrous ion-induced strand breaks in the plasmid pBR322 are mediated through hydrogen peroxide.
Działania biochem./fizjol.
One of the most commonly used cloning vectors, pBR322, confers resistance to ampicillin and tetracycline. The DNA sequence of the entire plasmid has been published.
This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives.
Komponenty
DNA is provided in a solution of 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
Inne uwagi
Accession number: J01749
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produkt powiązany
Numer produktu
Opis
Cennik
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Jörg Flemmig et al.
European biophysics journal : EBJ, 36(4-5), 377-384 (2006-10-19)
Ferrous ion-induced generation of single and multiple strand breaks in the DNA plasmid pBR322 induces the formation of two new plasmid forms with altered electrophoretic mobility. The yield of these plasmid forms, the circular relaxed and the linear forms, depended
Ferrous ion-induced strand breaks in the DNA plasmid pBR322 are not mediated by hydrogen peroxide
Flemmig J and Arnhold J
European Biophysics Journal, 36(4-5), 377-384 (2007)
Charles J Addison et al.
BioTechniques, 37(3), 376-378 (2004-10-09)
Transformation of Escherichia coli plays an important role in recombinant DNA technology. Most current transformation protocols require that the cells be treated to attain a particular physiological state known as "competence," and this makes transformation procedures lengthy and arduous. Here
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