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CHP1

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Imprint® Chromatin Immunoprecipitation Kit

Complete ChIP reaction in 6 hours in flexible strip well format

Synonim(y):

Immunoprecypitacja chromatyny

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About This Item

Kod UNSPSC:
41116158
NACRES:
NA.54

temp. przechowywania

−20°C

Powiązane kategorie

Opis ogólny

Imprint Chromatin Immunoprecipitation Kit provides a complete solution for Chromatin Immunoprecipitation including columns and reagents for DNA purification and an integrated protocol for ChIP DNA amplification with our GenomePlex® Whole Genome Amplification Kit (WGA2) . The flexible format allows for immunoprecipitation and purification of DNA from mammalian cells or tissue in a convenient strip-well format.

For Frequently Asked Questions about this kit, please see ChIP Troubleshooting Questions.

Zastosowanie

The Imprint Chromatin Immunoprecipitation Kit is a flexible, rapid and complete method for investigating protein-DNA interactions by chromatin immunoprecipitation (ChIP).
  • Suitable for downstream applications
  • Individual target characterization to genome-wide profiling techniques
  • Characterization of signal transduction pathways
  • Verification of ChIp-chIP and ChIP-seq data

Cechy i korzyści

  • Fast—Total protocol time of less than 6 hours making the Imprint kit the fastest on the market
  • Sensitive—As few as 10,000 cells required for each ChIP sample
  • Convenient—Fewest steps of any available ChIP protocol
  • Flexible—Protocols for cells or tissue, and convenient strip-well format for high-throughput applications
  • Complete—Includes columns and reagents for DNA purification as well as an integrated protocol for amplification with the GenomePlex technology

Informacje prawne

GenomePlex is a registered trademark of Takara Bio USA, Inc.
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
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Elementy zestawu są też dostępne oddzielnie

Numer produktu
Opis
Karta charakterystyki
  • C2112Column Preparation SolutionKarta charakterystyki
  • P4850Proteinase K from Tritirachium album, buffered aqueous glycerol solution, for molecular biology, ≥800 units/mLKarta charakterystyki
  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionKarta charakterystyki

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H225,H290,H302,H315,H319,H334,H336,H412

Klasyfikacja zagrożeń

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organy docelowe

Central nervous system

Kod klasy składowania

3 - Flammable liquids

Temperatura zapłonu (°F)

53.6 °F - closed cup

Temperatura zapłonu (°C)

12 °C - closed cup

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Ghazala Begum et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 26(4), 1694-1703 (2012-01-10)
Undernutrition during pregnancy is implicated in the programming of offspring for the development of obesity and diabetes. We hypothesized that maternal programming causes epigenetic changes in fetal hypothalamic pathways regulating metabolism. This study used sheep to examine the effect of
Poulomi Banerjee et al.
Journal of molecular medicine (Berlin, Germany), 94(12), 1397-1409 (2016-08-19)
Epithelial-mesenchymal transition (EMT) is one of the key biological phenomena behind cancer and metastasis. Clinical studies suggest that patients undergoing metformin therapy are less predisposed to cancer but the underlying mechanism is far from clear. Given that metformin also acts
Lucie Hyrsova et al.
British journal of pharmacology, 173(10), 1703-1715 (2016-02-28)
The organic cation transporter 1 (OCT1) transports cationic drugs into hepatocytes. The high hepatic expression of OCT1 is controlled by the HNF4α and USF transcription factors. Pregnane X receptor (PXR) mediates induction of the principal xenobiotic metabolizing enzymes and transporters
Thomas Palm et al.
Nucleic acids research, 41(6), 3699-3712 (2013-02-12)
Stem cell fate decisions are controlled by a molecular network in which transcription factors and miRNAs are of key importance. To systemically investigate their impact on neural stem cell (NSC) maintenance and neuronal commitment, we performed a high-throughput mRNA and
Ulrich Deuschle et al.
International journal of cancer, 136(11), 2693-2704 (2014-11-05)
The nuclear bile acid receptor Farnesoid X receptor (FXR) is strongly expressed in liver and intestine, controls bile acid and lipid homeostasis and exerts tumor-protective functions in liver and intestine. Histidine-rich glycoprotein (HRG) is an abundant plasma protein produced by
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Produkty

Histone Modification and Chromatin Remodeling | Epigenetics

Epigenetic modifications are thought to occur through two key interconnected processes—DNA methylation and the covalent modification of histones.

Protokoły

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

Analiza danych ChIP-qPCR

Immunoprecypitacja chromatyny qPCR do badania regulacji genów w różnych warunkach.

Imprint® Chromatin Immunoprecipitation Kit (CHP1) Protocol

The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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