MEF-1

98061101, mouse embryo, Fibroblast-like

• Kod UNSPSC: 41106514

Właściwości

• product name: MEF-1, 98061101
• pochodzenie biologiczne: mouse embryo
• tryb wzrostu: Adherent
• kariotyp: Not specified
• morfologia: Fibroblast-like
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Mouse embryo fibroblast, SV40 - transformed

Opis linii komórkowej

The cell line MEF-1 was established by transfection of mouse embryo fibroblasts ((C57 BL/6 x 129) F1) with a SV40 coding plasmid. MEF-1 express the wild-type for low density lipoprotein receptor related protein (LRP). Together with the cell lines PEA10 (Sigma Catalogue number. 97040310) and PEA13 (Sigma Catalogue number. 97040311), which express heterozygous and homozygous forms of LPR, an experimental system is available for the analysis of cellular uptake of functionally diverse ligands and the effect of LPR - deficiency. This is of importance since LRP - deficient mouse embryos die early during gestation.

Zastosowanie

Study of LRP and LRP-deficiency

pożywka hodowlana

DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:6 to 1:10 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

Inne uwagi

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