Eos-HL-60

96100920, human blood, Lymphoblastoid

• Kod UNSPSC: 41106514

Właściwości

• product name: Eos-HL-60, 96100920
• pochodzenie biologiczne: human blood
• tryb wzrostu: Suspension
• kariotyp: Not specified
• morfologia: Lymphoblastoid
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Caucasian promyelocytic leukaemia

Opis linii komórkowej

Eos-HL-60 is a variant of the promyelocytic cell line HL60 established by sub-cloning in methylcellulose. Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.Eos-HL-60 and HL-60 have been shown to be identical genetically by STR profiling

Zastosowanie

Study of production and function of human eosinophils, analysis of differentiation.

Profil DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 13,14
D13S317: 8,11
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 8
TPOX: 8,11
vWA: 16,17

pożywka hodowlana

IDMEM + 2mM Glutamine + 0.5mM Butyric acid + 10% Foetal Bovine Serum (FBS); pH7.6; add Butyric acid to the medium just before use.

Rutyna subkultury

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Inne uwagi

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