AFRC 17D1

94120825, Application: Fusion partner for hybridoma production

• Kod UNSPSC: 41106514

Właściwości

• pochodzenie biologiczne: Horse x Mouse/Horse Hybridoma
• ImmunoDon: Horse peripheral lymphocytes
• Szpiczak: NS0
• Reaktywność: Application: Fusion partner for hybridoma production
• metody: cell culture | mammalian: suitable
• izotyp: Not specified
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Horse peripheral blood lymphocytes x Mouse NS0

Opis linii komórkowej

The heterohybridoma AFRC 17D1 was obtained by fusion of lymphocytes from ponies immunised with the influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)) with mouse myeloma NS0 cells. The hybridoma was made aminopterin-sensitive by selective growth in 8-azaguanine. This non-Ig-secreting cell line was used as fusion partner for the establishment of equine-mouse heterohybidomas.

Immunogen

Influenza A equine 2 virus

Zastosowanie

Fusion partner for hybridoma production

pożywka hodowlana

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

Rutyna subkultury

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

Inne uwagi

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