CESS

92110206, human blood, Lymphoblast

• Kod UNSPSC: 41106514

Właściwości

• product name: CESS, 92110206
• pochodzenie biologiczne: human blood
• tryb wzrostu: Suspension
• kariotyp: Not specified
• morfologia: Lymphoblast
• produkty: Surface Immunoglobulin ( Ig), MHC Class II
• receptory: Fc, complement
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human lymphoblast

Opis linii komórkowej

Derived from a patient with myelomonocytic leukaemia and subsequently exposed to Epstein Barr Virus (EBV). This line is EBV positive and therefore presumably derived from a normal B lymphocyte. This cell line is also positive for surface Ig, Fc receptor, complement receptor, surface Ia and EBNA. Responds to T cell replacement factor by increasing the number of IgG secreting cells. The cells should be handled under laboratory containment level 2.

Zastosowanie

T cell replacement factor (TRF) assays, B cell differentiation studies

Profil DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,9
vWA: 16,17

pożywka hodowlana

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Maintain cultures between 2-9x100,000 cells/ml; 5% CO₂; 37°C.

Inne uwagi

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