A2058

91100402, human skin (lymph node metastasis), Epithelial

• Synonim(y): A2058 Cell Line
• Kod UNSPSC: 41106514

Właściwości

• product name: A2058, 91100402
• pochodzenie biologiczne: human skin (lymph node metastasis)
• tryb wzrostu: Adherent
• kariotyp: Not specified
• morfologia: Epithelial
• produkty: Not specified
• receptory: Nerve growth factor (NGF), Laminin
• metody: cell culture | mammalian: suitable
• powiązane choroby: metastasis
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human caucasian metastatic melanoma

Opis linii komórkowej

This cell line has been isolated from a lymph node metastasis from a 43 year old male patient with malignant melanoma. Due to their highly invasive properties, the cells can be used as a source of cellular invasion associated proteins e.g. collagen type IV collagenase, tissue inhibitor of metalloproteinase-2 and autocrine motility factor.

pożywka hodowlana

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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