BCL1 Clone CW13.20-3B3

90061904, mouse blood, Lymphoblast

• Kod UNSPSC: 41106514

Właściwości

• product name: BCL1 Clone CW13.20-3B3, 90061904
• pochodzenie biologiczne: mouse blood
• tryb wzrostu: Suspension
• kariotyp: Not specified
• morfologia: Lymphoblast
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Mouse BALB/c B cell leukaemia

Opis linii komórkowej

Derived from BCL1 by limiting dilution and does not spontaneously secrete IgM when cultured in pre-tested batches of FBS. The line is useful for studying soluble factors involved in B-cell differentiation.

Zastosowanie

B-cell differentiation studies

pożywka hodowlana

RPMI 1640 + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cells attach lightly to surface which should be tapped sharply to dislodge the cells. Do not allow cells to become confluent, subculture at 75% to 90% confluency; free-floating aggregates acc

Inne uwagi

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