RAG

89040605, mouse kidney, Ameboid-like

• Kod UNSPSC: 41106514

Właściwości

• product name: RAG, 89040605
• pochodzenie biologiczne: mouse kidney
• tryb wzrostu: Adherent
• kariotyp: Modal no. 68, hypertriploid
• morfologia: Ameboid-like
• produkty: ES-2
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Mouse BALB/c renal adenocarcinoma

Opis linii komórkowej

Derived from the original Renal-2a BALB/c renal adenocarcinoma. Cells produce the unique kidney specific esterase ES-2 and have been used in somatic cell hybridisation studies because of their azaguanine resistant properties.

Zastosowanie

Hybridisation, resistance and tumourigenicity studies

pożywka hodowlana

EMEM (HBSS) + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Inne uwagi

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