AFRC MAC 77

Reactivity: Anti-Nitrate Reductase, 85102262

• Kod UNSPSC: 41106514

Właściwości

• pochodzenie biologiczne: Rat x Rat Hybridoma
• ImmunoDon: F344 rat spleen
• Szpiczak: Y3Ag1.2.3
• Reaktywność: Anti-Nitrate Reductase
• metody: cell culture | mammalian: suitable
• izotyp: IgG2b
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Rat Y3Ag1.2.3 x Rat F344 spleen

Opis linii komórkowej

The antibody specific for NADH nitrate reductase and inhibits the reduction of nitrate to nitrite using NADH as electron donor. It also inhibits the reduction of nitrate using flavin nucleotides (FMNH2 or FADH2) and methyl viologen. The antibody is directed against NADH-NR of mono and dicotyledons.

Immunogen

Nitrate reductase purified from the leaves of 6 to 8 week old spinach plants - Spinacea oleracea l

pożywka hodowlana

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Rutyna subkultury

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Inne uwagi

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