Sf21

5022801, insect ovary, Spherical

• Kod UNSPSC: 41106514

Właściwości

• product name: Sf21, 05022801
• pochodzenie biologiczne: insect ovary
• tryb wzrostu: Adherent
• kariotyp: Not specified
• morfologia: Spherical
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Spodoptera frugiperda pupal ovarian tissue

Opis linii komórkowej

Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems. They were derived at the USDA Insect Pathology Laboratory from a cell line originating from pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. Sf21 cells have greater range in size and have a doubling time of 24 hr compared to 48-72 hr for Sf9 cells.

Zastosowanie

Protein expression using the Baculovirus vector system

pożywka hodowlana

SF90011 + 10% Foetal Bovine Serum (FBS)

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x10,000 cells/cm²; 28 C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, centrifuge suspension and resuspend cell pellet in fresh medium.

Inne uwagi

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