C5992

Anti-Cytokeratin, pan antibody, Mouse monoclonal

clone PCK-26, purified from hybridoma cell culture

• Synonim(y): Monoclonal Anti-pan-Cytokeratin, Anti-Cytokeratin, Anti-Cytokeratin - Monoclonal Anti-Cytokeratin, pan antibody produced in mouse
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 200
• białko sprzężone: unconjugated
• forma przeciwciała: purified from hybridoma cell culture
• rodzaj przeciwciała: primary antibodies
• klon: PCK-26, monoclonal
• Postać: buffered aqueous solution
• reaktywność gatunkowa: human, chicken, snake, hamster, pig, goat, feline, bovine, carp, rat, rabbit, canine, lizard, sheep, mouse, guinea pig
• opakowanie: antibody small pack of 25 μL
• stężenie: ~1.5 mg/mL
• metody: dot blot: suitable; immunocytochemistry: suitable; immunohistochemistry: 10-20 μg/mL using human placenta or skin; western blot: suitable
• izotyp: IgG1
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: bovine ... Krt1(100301161) ; dog ... Krt1(444857) ; human ... KRT1(3848) , KRT1(3848) , KRT1(3848) , KRT5(3852) , KRT5(3852) , KRT5(3852) , KRT6A(3868) , KRT6A(3868) , KRT6A(3868) , KRT6B(3854) , KRT6B(3854) , KRT6B(3854) , KRT8(3856) , KRT8(3856) , KRT8(3856) ; mouse ... KRT1(16678) , KRT1(16678) , KRT1(16678) , Krt1(16678) , Krt5(110308) , Krt5(110308) , Krt5(110308) , Krt6a(16687) , Krt6a(16687) , Krt6a(16687) , Krt6b(16688) , Krt6b(16688) , Krt6b(16688) , Krt8(16691) , Krt8(16691) , Krt8(16691) ; rat ... Krt1(300250) , Krt2-5(369017) , Krt2-5(369017) , Krt2-5(369017) , Krt2-8(25626) , Krt2-8(25626) , Krt2-8(25626)

Opis

Opis ogólny

Monoclonal Anti-Pan Cytokeratin (mouse IgG1 isotype) is derived from the PCK-26 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a cytokeratin preparation from human epidermis. Cytokeratins are a group of at least 29 different proteins. They are characteristic of epithelial and trichocytic cells. Cytokeratin peptide 1 (68 kDa) is expressed together with cytokeratin 10 in the suprabasal cell layers or the differentiation compartment of the epidermis. Its expression increases with epidermal maturation and is modified post translationally in terminally differentiated keratinocytes of the stratum corneum. Cytokeratin peptide 5 (58 kDa) is the primary type II keratin in stratified epithelia, while cytokeratin type 8 (52 kDa) is a major type II keratin in simple epithelia. Cytokeratin 6 (56 kDa) is a "hyperproliferation" cytokeratin expressed in tissues with natural or pathological high turnover. Monoclonal antibodies to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification.

Specyficzność

The antibody recognizes an epitope located on the Type II cytokeratins 1, 5, 6, and 8. PCK-26 is a broad spectrum antibody which reacts specifically with a variety of normal, reactive, and neoplastic epithelial tissues. The antibody reacts with simple, cornifying, and non-cornifying squamous epithelia and pseudostratified epithelia.

Immunogen

cytokeratin from human epidermis.

Zastosowanie

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Monoclonal Anti-Cytokeratin, pan antibody produced in mouse has been used in:

• immunoblotting
• immunohistochemistry
• Immunocytochemistry
• dot blotting

Działania biochem./fizjol.

Cytokeratin facilitates the typing of normal, metaplastic, and neoplastic cells and may aid in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas, and neural tumors. It is also useful in detecting micrometastases in lymph nodes and other tissues, and for determining the origin of poorly differentiated tumors.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable