C-39606

Renal Epithelial Cell Growth Medium 2

SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

• Synonim(y): Recommended with C-26230
• Kod UNSPSC: 12352207
• NACRES: NA.71

Właściwości

• Formularz: aqueous solution
• opakowanie: pkg of 1 vial
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −20°C

Opis

Opis ogólny

Renal Epithelial Cell Growth Medium 2 SupplementMix (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-26230

Zastosowanie

Renal Epithelial Cell Growth Medium 2 is an optimized low-serum (5% V/V) medium that additionally contains Epinephrine. The combination of low serum and selected growth factors ensures successful growth in vitro making the Renal Epithelial Cell Growth Medium 2 the first choice for PromoCell Renal Epithelial Cells. The Renal Epithelial Cell Growth Medium 2 is optimized for primary human cells, but can also be used for porcine and mouse renal epithelial cells.Recommended for:Human Renal Epithelial Cells (HREpC)Human Renal Cortical Epithelial Cells (HRCEpC)

Komponenty

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Jakość

All lots of PromoCell Renal Epithelial Cell Media are subjected to comprehensive quality control tests using primary human renal epithelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested renal epithelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Uwaga dotycząca przygotowania

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / ml Epidermal Growth Factor (recombinant human): 10 ng / mlInsulin (recombinant human): 5 µg / mlHydrocortisone: 36 ng / mlEpinephrine: 0.5 µg / mlTriiodo-L-thyronine: 4 pg / mlTransferrin (recombinant human): 5 µg / ml

Przechowywanie i stabilność

Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Rutyna subkultury

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3