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Key Documents

C-39320

Sigma-Aldrich

Fibroblast Growth Medium 2

SupplementPack containing all media supplements as individual vials, 1 Pack for 500 ml

Synonim(y):

Recommended with C-23220

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About This Item

Kod UNSPSC:
12352207
NACRES:
NA.71

Postać

liquid

opakowanie

pkg of 1 kit

metody

cell culture | mammalian: suitable

Warunki transportu

dry ice

temp. przechowywania

−20°C

Opis ogólny

Fibroblast Growth Medium 2 SupplementPack (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-23220

Zastosowanie

Fibroblast Growth Medium 2 is a low-serum medium and has been developed for the in vitro cultivation of adult Human Fibroblasts. The medium is optimized for primary human cells, but can also be used for bovine, porcine, and rat fibroblasts, as well as fibroblast cell lines.Recommended for:Normal Human Dermal Fibroblasts (NHDF), adult donorHuman Pulmonary Fibroblasts (HPF)Human Aortic Adventitial Fibroblasts (HAoAF)Human Uterine Fibroblasts (HUF)

Komponenty

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Jakość

All lots of PromoCell Fibroblast Growth Medium 2 are subjected to comprehensive quality control tests using primary human adult fibroblasts. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested adult fibroblasts. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Uwaga dotycząca przygotowania

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.02 ml / mlBasic Fibroblast Growth Factor (recombinant human): 1 ng / ml Insulin: 5 µg / ml

Przechowywanie i stabilność

Store the SupplementPack at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Rutyna subkultury

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polecane

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Opis
Cennik

Kod klasy składowania

10 - Combustible liquids


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Produkty

Primary human fibroblasts and growth medium for the in vitro cultivation of juvenile and adult fibroblasts. Protocols for handling, cell passaging, media, and product use.

Pierwotne ludzkie fibroblasty i pożywka wzrostowa do hodowli in vitro młodych i dorosłych fibroblastów. Protokoły obsługi, pasażowania komórek, pożywki i stosowania produktu.

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