C213100

DMT-2′O-Methyl-rC(ac) Phosphoramidite

• Synonim(y): N-acetyl-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-methyl-cytidine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]
• Wzór empiryczny (zapis Hilla): C₄₂H₅₂N₅O₉P
• Numer CAS: 199593-09-4
• Masa cząsteczkowa: 801.86
• Numer MDL: MFCD12912403
• Kod UNSPSC: 41116105
• NACRES: NA.51

Właściwości

• pochodzenie biologiczne: non-animal source (no BSE/TSE risk)
• Poziom jakości: 300
• linia produktu: Proligo Reagents
• Próba: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• Formularz: powder
• metody: oligo synthesis: suitable
• zanieczyszczenia: ≤0.1% single unspecified Impurity (reversed phase HPLC); ≤0.3% mC2 (reversed phase HPLC, Hydrolysate); ≤0.3% mC3 (reversed phase HPLC, DMT-rC(ac)me); ≤0.3% mC4 (reversed phase HPLC, DMT-rC(ac)me-Ac); ≤0.3 wt. % water content (Karl Fischer); ≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR); ≤1.0% mC1 (reversed phase HPLC, DMT-rC(ac)me-DMT); ≤3 wt. % residual Solvent content
• kolor: white to off-white
• λ: conforms (UV/VIS Identity)
• przydatność: conforms to structure for H-NMR; conforms to structure for LC-MS
• profil nukleozydowy: base: cytidine; base protecting group: acetyl; 2' protecting group: methyl; 5' protecting group: DMT; deprotection: fast/standard
• temp. przechowywania: 2-8°C
• ciąg SMILES: P(N(C(C)C)C(C)C)(O[C@@H]1[C@H](O[C@H](C1OC)N5C=CC(=NC5=O)NC(=O)C)COC(c4ccc(cc4)OC)(c3ccc(cc3)OC)c2ccccc2)OCCC#N
• InChI: 1S/C42H52N5O9P/c1-28(2)47(29(3)4)57(54-26-12-24-43)56-38-36(55-40(39(38)52-8)46-25-23-37(44-30(5)48)45-41(46)49)27-53-42(31-13-10-9-11-14-31,32-15-19-34(50-6)20-16-32)33-17-21-35(51-7)22-18-33/h9-11,13-23,25,28-29,36,38-40H,12,26-27H2,1-8H3,(H,44,45,48,49)/t36-,38-,39?,40-,57?/m1/s1
• Klucz InChI: WNWUMIPFLOKTEZ-IXZONTHDSA-N

Opis

Opis ogólny

DMT-2′O-Methyl-rC(ac) Phosphoramidite belongs to the group of 2′O-Methyl RNA Phosphoramidites. 2′O-Methyl RNA monomers are compatible with fast deprotection schemes that are based on the application of aliphatic amines, such as methylamine. 2′O-Methyl RNA is a nucleic acid analog that is characterized by the exceptional hybridization properties that it imparts with complementary DNA or RNA, as well as increased stability against enzymatic degradation compared to natural nucleic acids. 2′O-Methyl RNA monomers feature methoxy groups at the 2′-position. The methoxy groups are perfectly stable in all conditions employed in the assembly of oligonucleotides by automated phosphoramidite synthesis, and in all standard alkaline deprotection conditions.

Zastosowanie

DMT-2′O-Methyl-rC(ac) Phosphoramidite can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance.

Inne uwagi

It has widespread applications in the fields of:

• Diagnostic probes
• Aptamer and ribozyme development
• Mixed 2′O-Methyl-RNA/DNA antisense molecules

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable