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BCK555-IV-HTS-S

Sigma-Aldrich

In Vivo EdU HTS 2x96 Kit 555

sufficient for 200 assays

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About This Item

Kod UNSPSC:
12161503
NACRES:
NA.22

fluorescencja

λex 546 nm; λem 579 nm

Warunki transportu

wet ice

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Opis ogólny

The Baseclick EdU In Vivo Kits provide a superior alternative to BrdU and [3H]thymidine assays for detection of cell proliferation in whole animals, showing no toxic effects. EdU (5-ethynyl-2′-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. In contrast to BrdU assays, the EdU In Vivo Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the EdU In Vivo Kits utilize click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol reduces both the total number of steps and significantly decreases the total amount of time. The simple click chemistry detection procedure is complete within 30 minutes and is compatible with multiplexing for content and context-rich results.The in vivo kits are designed in a sense that you combine one of the three baseclick EdU cell proliferation kits such as:

1. Imaging kit (BCK-IV-IM)
2. Flow cytometry kit (BCK-IV-FC)
3. High throughput screening – HTS kit (BCK-IV-HTS)

You can select the above kits with the dye of choice and the right EdU content for your animal model.

Depending on your animal model or the number of animals to be tested you can choose between three kit sizes S, M, and L with increasing EdU content, as indicated in the following table.

Kit Size Content of EdU
S 50 mg
M 500 mg
L 1000 mg

Zastosowanie

in Vivo, High Throughput Screening
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Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H302 + H332,H314,H340,H361,H412

Klasyfikacja zagrożeń

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Muta. 1B - Repr. 2 - Skin Corr. 1C

Zagrożenia dodatkowe

EUH032

Kod klasy składowania

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Maia Dorsett et al.
Genetics, 183(1), 233-247 (2009-07-15)
Germ-line stem cells are unique because they either self-renew through mitosis or, at a certain frequency, switch to meiosis and produce gametes. The switch from proliferation to meiosis is tightly regulated, and aberrations in switching result in either too little
Adrian Salic et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(7), 2415-2420 (2008-02-15)
We have developed a method to detect DNA synthesis in proliferating cells, based on the incorporation of 5-ethynyl-2'-deoxyuridine (EdU) and its subsequent detection by a fluorescent azide through a Cu(I)-catalyzed [3 + 2] cycloaddition reaction ("click" chemistry). Detection of the

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