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B9139

Sigma-Aldrich

Biotin-dextran

mol wt 10,000, Lysine-fixable

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About This Item

Numer MDL:
Kod UNSPSC:
12352201
eCl@ss:
34058011
NACRES:
NA.25

białko sprzężone

biotin conjugate

Poziom jakości

Formularz

powder

masa cząsteczkowa

10,000

zakres etykietowania

~2 mol per mol

kolor

white

rozpuszczalność

H2O: soluble 50 mg/mL, clear, colorless to faintly yellow

temp. przechowywania

2-8°C

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Zastosowanie

Biotin-dextran is used in tissue staining to prepare neurons for detection by avidin-conjugated detection systems. It is taken up by neuronal processes and transported bi-directionally. It infiltrates axon boutons in the anterograde direction and dendritic processes in the retrograde direction. Staining can be observed in fixed and sectioned tissue from two days to two weeks after biotin-dextran is injected into the brain or spinal cord. It can also be applied to cut nerve tracts. Biotinylated neurons are detected by either light or electron microscopy following incubation with avidin-horseradish peroxidase conjugate and the electron-dense substrate 3,3′-diaminobenzidine (DAB).

Inne uwagi

To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

C Brösamle et al.
Journal of neurocytology, 29(7), 499-507 (2001-03-30)
The corticospinal tract (CST) of the rat is a widely used model system in developmental, physiological, and regeneration studies. The CST of the rat consists of a main tract, that runs in the dorsomedial funiculus and several minor components. We
Karen M Fisher et al.
The Journal of comparative neurology, 526(15), 2373-2387 (2018-07-18)
The corticospinal tract (CST) forms the major descending pathway mediating voluntary hand movements in primates, and originates from ∼nine cortical subdivisions in the macaque. While the terminals of spared motor CST axons are known to sprout locally within the cord
Giam S Vega-Meléndez et al.
Journal of neuroscience research, 92(1), 13-23 (2013-10-30)
Neurotrophins such as ciliary neurotrophic factor (CNTF) and brain-derived neurotrophic factor (BDNF) and growth factors such as fibroblast growth factor (FGF-2) play important roles in neuronal survival and in axonal outgrowth during development. However, whether they can modulate regeneration after
Jia Liu et al.
Journal of the neurological sciences, 325(1-2), 127-136 (2013-01-16)
The stem cell-based experimental therapies are partially successful for the recovery of spinal cord injury (SCI). Recently, acellular spinal cord (ASC) scaffolds which mimic native extracellular matrix (ECM) have been successfully prepared. This study aimed at investigating whether the spinal
Victoria L Cressman et al.
The Journal of comparative neurology, 518(14), 2693-2709 (2010-05-28)
Transformations in affective and social behaviors, many of which involve amygdalar circuits, are hallmarks of adolescence in many mammalian species. In this study, using the rat as a model, we provide the first evidence that afferents of the basal amygdala

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