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Key Documents

B5283

Sigma-Aldrich

Anti-Rabbit IgG (γ-chain specific)−Biotin antibody, Mouse monoclonal

clone RG-96, purified from hybridoma cell culture

Synonim(y):

Monoclonal Anti-Rabbit IgG (γ-chain specific)

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.46

pochodzenie biologiczne

mouse

Poziom jakości

200

białko sprzężone

biotin conjugate

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

secondary antibodies

klon

RG-96, monoclonal

Postać

buffered aqueous solution

reaktywność gatunkowa

rabbit

spodziewany brak reakcji z

goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken

metody

direct ELISA: 1:150,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000
western blot (chemiluminescent): 1:200,000-1:400,000

izotyp

IgG1

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

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Opis ogólny

IgG (immunoglobulin G) antibody is secreted by B cells and is found in blood and extracellular fluids and is enriched in mother′s milk. The variable region of IgG antibody is specific to antigens and is highly conserved.
The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.

Zastosowanie

Monoclonal Anti-Rabbit IgG (γ-chain specific)-Biotin is suitable for ELISA at a working dilution of 1:150,000. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a dilution of 1:1000. The antibody was used in β-catenin ELISA at a dilution of 1:2000.
Anti-Rabbit IgG (γ-chain specific)-Biotin antibody, Mouse monoclonal has been used in β-Catenin enzyme immunoassay. It has also been used in immunohistochemistry for examining histology of mammary glands.

Działania biochem./fizjol.

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Rabbit IgG (γ-chain specific) antibody allows for the binding of various labels such as avidin or streptavidin. IgG antibody has its function similar to IgM antibody in complement system activation.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

nwg

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

β-Catenin Signaling Promotes Proliferation of Progenitor Cells in the Adult Mouse Subventricular Zone.
Kazuhide A, et al.
Stem Cells, 25(11), 2827-2836 (2007)
Miguel A Ortega et al.
Cells, tissues, organs, 206(4-5), 272-282 (2019-06-17)
Chronic venous disorder (CVeD), is a disorder in which there is a modification in the conditions of blood return to the heart. The disorder may arise from incompetent valves and the resultant venous reflux (chronic venous insufficiency, CVI). The economic
Regulatory function of whey acidic protein in the proliferation of mouse mammary epithelial cells in vivo and in vitro.
Nukumi N, et al.
Developmental Biology, 274(1), 31-44 (2004)
Retained features of embryonic metabolism in the adult MRL mouse.
Naviaux RK, et al.
Molecular Genetics and Metabolism, 96(3), 133-144 (2009)
Yoann Aldon et al.
Cell reports, 24(12), 3324-3338 (2018-09-21)
The HIV-1-envelope glycoprotein (Env) is the main target of antigen design for antibody-based prophylactic vaccines. The generation of broadly neutralizing antibodies (bNAb) likely requires the appropriate presentation of stabilized trimers preventing exposure of non-neutralizing antibody (nNAb) epitopes. We designed a
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