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Merck

A9205

Sigma-Aldrich

Bovine Serum Albumin solution

30% in saline, fatty acid free, aseptically filled

Synonim(y):

Albumin solution from bovine serum, BSA solution

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About This Item

Numer CAS:
Numer MDL:
Kod UNSPSC:
12352202
NACRES:
NA.27

pochodzenie biologiczne

bovine

Poziom jakości

sterylność

aseptically filled

Próba

28-32% protein basis (biuret)

Postać

solution

oczyszczone przez

cold ethanol fractionation

nie zawiera

stabilizer or preservative

pochodzenie

USA origin

stężenie

28-32% protein (biuret)
30% in saline

free fatty acid content

≤0.01%

metody

ELISA: suitable
electrophoresis: suitable
western blot: suitable

zanieczyszczenia

≤0.01% free fatty acid

numer dostępu UniProt

obecność zanieczyszczeń

BT virus, none detected
VSV virus, none detected

temp. przechowywania

2-8°C

informacje o genach

bovine ... ALB(280717)

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Opis ogólny

Bovine Serum Albumin (BSA) has three homologous domains, each subdivided it two domain (A and B). It is a α-helical globular protein and shares structural features with human serum albumin.

Zastosowanie

Bovine Serum Albumin solution has been used:
  • as Dulbecco′s modified Eagle′s medium supplement for culturing HepG2 human liver cancer cells
  • as a component of hepatocyte recovery medium
  • as a component of fusion and activation buffers for in vitro reconstructed sheep embryos

Działania biochem./fizjol.

Bovine Serum Albumin (BSA) is the major protein present in blood and has calcium binding sites and regulates metal homeostasis. It is a crucial component of the cell culture medium and promotes embryonic stem cells (hESC) differentiation. Due to its high solubility, low cost and purity it is widely used in cosmetic and pharmaceutical industry. BSA is used as a blocking agent used in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates.

Uwaga dotycząca przygotowania

Initial fractionation by cold alcohol precipitation
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Effects of different states of sheep fetal fibroblasts as donor cells on the early development in vitro of reconstructed sheep embryos
Wang H, et al.
Science in China. Series C, Life Sciences / Chinese Academy of Sciences, 50(2), 178-185 (2007)
Pilar Argente-Arizón et al.
Endocrinology, 159(1), 368-387 (2017-10-28)
Astrocytes participate in both physiological and pathophysiological responses to metabolic and nutrient signals. Although most studies have focused on the astrocytic response to weight gain due to high-fat/high-carbohydrate intake, surplus intake of a balanced diet also induces excess weight gain.
Yi Fan et al.
SLAS discovery : advancing life sciences R & D, 23(7), 742-750 (2018-06-07)
Enhancing antitumor activities of the human immune system is a clinically proven approach with the advent of monoclonal antibodies recognizing programmed cell death protein-1 (PD1) receptors on immune cell surfaces. Historically, using flow cytometry as a means to assess next-generation
Jing-Syuna Ruan et al.
The Journal of nutritional biochemistry, 59, 37-48 (2018-07-01)
Lipotoxicity is associated with a high level of fatty acid accumulation in pancreatic β-cells. An overload of free fatty acids contributes to pancreatic β-cell apoptosis and dysfunction. Insulin secretion involves sequential ionic events upon glucose stimulation. ATP sensitive potassium (KATP)
Down-regulation of the mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase gene by insulin: the role of the forkhead transcription factor FKHRL1
Nadal A, et al.
The Biochemical Journal, 366(1), 289-297 (2002)

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