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A7345
Anti-V5 Agarose Affinity Gel antibody produced in mouse
purified immunoglobulin, clone V5-10
Synonim(y):
Monoclonal Anti-V5
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About This Item
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
białko sprzężone
agarose conjugate
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
V5-10, monoclonal
klasy chemiczne analitów
proteins (V5)
metody
affinity chromatography: suitable
immunoprecipitation (IP): suitable
pojemność
2.5 nmol/mL, gel (V5 fusion protein)
Warunki transportu
wet ice
temp. przechowywania
2-8°C
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Opis ogólny
Anti-V5 Agarose Affinity Gel is made of purified immunoglobulin fraction of monoclonal anti-V5 isolated from ascites fluid of the V5-10 hybridoma coupled to cyanogen bromide-activated beaded agarose. The purified monoclonal antibody is immobilized on agarose.
The Anti-V5 Agarose Affinity Gel is composed of the monolonal anti-V5 antibody conjugated to cyanogen bromide-activated beaded agarose. The antibody recognizes the V5-tagged recombinant fusion proteins expressed in transfected mammalian cells.
Specyficzność
Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins
Zastosowanie
Anti-V5 Agarose Affinity Gel antibody produced in mouse has been used in immunoprecipitation and recombinant protein purification.
Działania biochem./fizjol.
Recombinant DNA technology enables the attachment of genes of interest to specific sequences or genes that can provide ′affinity handles′ designed to enable the selective identification and purification of the protein of interest. The addition of a V5 tag sequence (GKPIPNPLLGLDST) to a given gene creates a stable fusion product that does not appear to interfere with the bioactivity of the protein, or with the biodistribution of the tagged product. Many recombinant proteins have been engineered to express a short sequence derived from the V5 from simian virus known as V5-tag. Anti-“tag” agarose conjugates may facilitate the detection, isolation and purification of these proteins.
Postać fizyczna
Suspension of beaded agarose (1:1 v/v) in 0.01 M phosphate buffered saline, containing 15 mM sodium azide.
Przechowywanie i stabilność
For continuous use and extended storage, store at 2 °C to 8 °C. Do not freeze.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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