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Merck

A7089

Sigma-Aldrich

Accumax solution

sterile-filtered, suitable for cell culture

Synonim(y):

Cell detachment solution

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About This Item

Numer MDL:
Kod UNSPSC:
12352204
NACRES:
NA.75

sterylność

sterile-filtered

Poziom jakości

Postać

solution

stężenie

1 ×

metody

cell culture | mammalian: suitable
single cell analysis: suitable

pH

6.8-7.8

Warunki transportu

dry ice

temp. przechowywania

−20°C

Zastosowanie

TM=["Accumax"] solution has been used:
  • for detaching single walled carbon nanotubes from the extracellular matrix (ECM) in epithelial cells
  • for dissociation of retinal pigmented epithelial cells prior to passaging
  • for dissociation of islets from pancreas

Accumax has proven effective in dissociating clumped cells in suspension cultures of hybridomas, CHO, BHK, 293, COS and Sf9 cells. The solution performed extremely well in dissociating cell counting, viral transfection assays, cell sorting, flow cytometry, and bioreactor scale-up. It has also been used to create single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue, and the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware.

Działania biochem./fizjol.

Accumax is a cell detachment solution consisting of proteolytic and collagenolytic enzymes. Each bottle has 100 mL of 1x Accumax enzymes in Dulbecco′s PBS. There are no mammalian or bacterial-derived products found in Accumax.

Cechy i korzyści

Ready-to-use sterile liquid for in vitro cell applications

Przestroga

Accumax is stable when stored at -20°C. After thawing, the product can be stored for up to 2 months at 4°C.

Wariant wzoru

Prepared in Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4). Refer to Product Data Sheet on the website for product usage information.

Uwaga dotycząca przygotowania

This product is supplied as a sterile, ready-to-use frozen liquid. Accumax should be thawed at 37°C before use, and added at equal volume to a sample of clumped cells. Allow 5-10 minutes at 37°C for incubation and count cells as you would normally.

Informacje prawne

Accumax is a trademark of Innovative Cell Technologies, Inc.
This page may contain text that has been machine translated.

produkt powiązany

Numer produktu
Opis
Cennik

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration
Geng Z, et al.
PLoS ONE, 12(3), e0173575-e0173575 (2017)
Chronic beta-cell depolarization impairs beta-cell identity by disrupting a network of ca2+-regulated genes
Stancill JS, et al.
Diabetes, 66(8), 2175-2187 (2017)
Influence of single walled carbon nanotubes at subtoxical concentrations on cell adhesion and other cell parameters of human epithelial cells
Kaiser JP, et al.
Journal of King Saud University - Science, 25(1), 15-27 (2013)
Zhouhui Geng et al.
PloS one, 12(3), e0173575-e0173575 (2017-03-11)
Fidelity in pluripotent stem cell differentiation protocols is necessary for the therapeutic and commercial use of cells derived from embryonic and induced pluripotent stem cells. Recent advances in stem cell technology, especially the widespread availability of a range of chemically
Natalja Funk et al.
Scientific reports, 9(1), 4515-4515 (2019-03-16)
Mutations within Leucine-rich repeat kinase 2 (LRRK2) are associated with late-onset Parkinson's disease. The physiological function of LRRK2 and molecular mechanism underlying the pathogenic role of LRRK2 mutations remain uncertain. Here, we investigated the role of LRRK2 in intracellular signal

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