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Merck

A5463

Sigma-Aldrich

Anti-Acetylated Proteins antibody produced in rabbit

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

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About This Item

Numer MDL:
Kod UNSPSC:
12352203

pochodzenie biologiczne

rabbit

białko sprzężone

unconjugated

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

primary antibodies

klon

polyclonal

Postać

buffered aqueous solution

reaktywność gatunkowa

human, chicken, bovine

stężenie

~0.5 mg/mL

metody

indirect immunofluorescence: 1:1,000 using methanol/acetone-fixed chicken fibroblats cells
microarray: suitable
western blot: 1:1,000 using Whole cell extract of human epitheloid carcinoma HeLa cell line treated with sodium butyrate

Warunki transportu

dry ice

temp. przechowywania

−20°C

Opis ogólny

Protein acetylation on lysine is a reversible protein modification that regulates protein functions. Histone acetylation at N-epsilon-lysine residues can destabilize the nucleosome structure and render DNA more accessible to transcription factors. Protein acetylation controls the function of transcription factors such as p53, GATA-1, E2F, TFIIEb, TFIIF, and EKLF.
Staining of histone H4 in immunoblotting is specifically inhibited with acetylated histone H4 immunizing peptide (human, amino acids 1-20 with N-epsilon-acetylated lysines at positions 5, 8, 12, and 16). No inhibition with the non-acetylated histone H4 peptide (human, amino acids 1-20) is observed.
Reacts with several acetylated proteins (e.g., acetylated histone H4 [12 kDa], acetylated H1 and H2b and acetylated BSA). Does not recognize acetylated histone H3.

Immunogen

synthetic acetylated peptide corresponding to the N-terminus of human histone H4 (amino acids 1-20, with N-ε-acetylated lysines at positions 5,8,12 and 16) conjugated to KLH.

Zastosowanie

Rabbit anti-acetylated protein antibody can be used for microarrays, indirect immunofluorescence (1:1,000) and western blot (1:1,000) assays.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

nwg

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Stéphane Bourque et al.
The New phytologist, 192(1), 127-139 (2011-06-10)
• Plant resistance to pathogen attack is often associated with a localized programmed cell death called hypersensitive response (HR). How this cell death is controlled remains largely unknown. • Upon treatment with cryptogein, an elicitor of tobacco defence and cell
J Boyes et al.
Nature, 396(6711), 594-598 (1998-12-22)
Modification of histones, DNA-binding proteins found in chromatin, by addition of acetyl groups occurs to a greater degree when the histones are associated with transcriptionally active DNA. A breakthrough in understanding how this acetylation is mediated was the discovery that
L Liu et al.
Molecular and cellular biology, 19(2), 1202-1209 (1999-01-16)
The p53 tumor suppressor protein is a sequence-specific transcription factor that modulates the response of cells to DNA damage. Recent studies suggest that full transcriptional activity of p53 requires the coactivators CREB binding protein (CBP)/p300 and PCAF. These coactivators interact

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