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Key Documents
A50120
DEAE–Sephadex®
Synonim(y):
Diethylaminoethyl Sephadex®
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About This Item
Polecane produkty
Postać
powder
Poziom jakości
metody
protein purification: suitable
macierz
cross-linked dextran
granulacja
40-125 μm (dry)
wielkość porów
~200,000 Da exclusion limit
pH
2—12
pojemność
3-4 meq/g binding capacity
Powiązane kategorie
Opis ogólny
A50120-100G′s updated product number is GE17-0180-01
A50120-500G′s updated product number is GE17-0180-02
A50120-500G′s updated product number is GE17-0180-02
Zastosowanie
DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins. In has been used to identify and label an insulin activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients.
Informacje prawne
Sephadex is a registered trademark of Cytiva
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zastąpiony przez
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
Certyfikaty analizy (CoA)
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Clovis R Nakaie et al.
Analytical biochemistry, 318(1), 39-46 (2003-06-05)
This report demonstrates that due to the presence of residual reactive sites in their matrices, classical diethylaminoethyl-attaching commercial anion-exchanger resins such as DEAE-MacroPrep and DEAE-Sephadex A50 supports can be used for peptide synthesis. Moreover, due to the high stability of
W Windig et al.
Developments in biological standardization, 47, 169-177 (1981-01-01)
Poliovirus suspensions produced in microcarrier culture of primary monkey kidney cells and purified by column chromatography on DEAE-sephadex have been tested for the presence of DEAE-polysaccharides (in essence DEAE-dextran) by pyrolysis mass spectrometry. Semi-quantitative evaluation of the pyrolysis mass spectra
B Ullman et al.
Biochimica et biophysica acta, 403(2), 393-411 (1975-10-22)
A phosphoprotein phosphatase which is active against chemically phosphorylated protamine has been purified about 500-fold from bovine adrenal cortex. The enzyme has a pH optimum between 7.5 and 8.0, and has an apparent Km for phosphoprotamine of about 50 muM.
Misook Kim et al.
Biochimica et biophysica acta, 1770(12), 1627-1635 (2007-10-09)
Two cysteine proteases, GP2 and GP3, have been isolated from ginger rhizomes (Zingiber officinale). GP2 is virtually identical to a previously identified ginger protease GPII [K.H. Choi, and R.A. Laursen, Amino-acid sequence and glycan structures of cysteine proteases with proline
M A Zaval'nyĭ et al.
Voprosy virusologii, (5)(5), 583-589 (1980-09-01)
Primary cells of chick embryos (CEC), new-born piglet kidneys (NPK), and green monkey kidney (GMK) obtained by the conventional method of tissue trypsinization were shown to grow satisfactorily on microcarriers of various types. CEC formed a confluent monolayer on Supperbead
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