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A2179

Sigma-Aldrich

Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonim(y):

Goat Anti-Mouse IgG (Fab specific)–AP

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About This Item

Numer MDL:
MFCD00162620
Kod UNSPSC:
12352203
NACRES:
NA.46

pochodzenie biologiczne

goat

białko sprzężone

alkaline phosphatase conjugate

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

secondary antibodies

klon

polyclonal

Postać

buffered aqueous solution

reaktywność gatunkowa

mouse

spodziewany brak reakcji z

horse, human, bovine

metody

direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25
western blot (chemiluminescent): 1:80,000

Warunki transportu

wet ice

temp. przechowywania

2-8°C

docelowa modyfikacja potranslacyjna

unmodified

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Opis ogólny

Immunoglobulins (Igs) belongs to the immunoglobulin super-family. Each immunoglobin have two heavy (H) and two light (L) chains held together by disulfide linkages.

Immunogen

Purified mouse IgG, Fab fragment

Zastosowanie

Alkaline phosphatase-conjugated goat anti-mouse IgG (Fab specific) was used as the secondary antibody in ELISA assays to measure the reacitivity of mABs to thyrogobulin peptides. Absorbance was measured at 405 nm.
Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in goat has been used in:
  • western blotting
  • immunoblotting
  • enzyme linked immunosorbent assay (ELISA)
  • immunohistochemistry

Działania biochem./fizjol.

Digestion of IgG by papain results in generation of fragment antigen binding (Fab) comprising of one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallisable (fc), comprises of the H chain constant region. IgG antibody have enormous therapeutic potential and the Fc are is for the development of therapeutic antibody. Although the antibody site is located in the terminal end of the human IgG molecule (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibodies directed to Fc), passage through placental membrane and Staphylococcus protein A binding.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice .

Inne uwagi

Antibody adsorbed with bovine, equine and human serum proteins.

Postać fizyczna

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.

Uwaga dotycząca przygotowania

Adsorbed to reduce background staining with bovine, horse, or human samples.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

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Odwiedź Bibliotekę dokumentów

M K Magnusson et al.
Cancer gene therapy, 14(5), 468-479 (2007-02-03)
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Characterization of a Monoclonal Antibody and Its Single-Chain Antibody Fragment Recognizing the Nucleoside Triphosphatase/Helicase Domain of the Hepatitis C Virus Nonstructural 3 Protein
Zhang, Z., X., et al.
Clinical and Vaccine Immunology : CVI, 7(1), 58-63 (2000)
Laszlo L P Hosszu et al.
Communications biology, 3(1), 402-402 (2020-07-31)
Prion diseases, a group of incurable, lethal neurodegenerative disorders of mammals including humans, are caused by prions, assemblies of misfolded host prion protein (PrP). A single point mutation (G127V) in human PrP prevents prion disease, however the structural basis for
Janine R Roper et al.
The Journal of biological chemistry, 280(20), 19728-19736 (2005-03-16)
Galactose metabolism is essential in bloodstream form Trypanosoma brucei and is initiated by the enzyme UDP-Glc 4'-epimerase. Here, we show that the parasite epimerase is a homodimer that can interconvert UDP-Glc and UDP-Gal but not UDP-GlcNAc and UDP-GalNAc. The epimerase
Manipulation of offspring number and size: benefits of large body size at birth depend upon the rearing environment
Oksanen TA, et al.
The Journal of animal ecology, 72(2), 321-330 (2003)
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