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352-05A

Sigma-Aldrich

Human Pulmonary Artery Smooth Muscle Cells: HPASMC, adult

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About This Item

Kod UNSPSC:
41106514
NACRES:
NA.81

pochodzenie biologiczne

human intrathoratic artery (normal)

Poziom jakości

opakowanie

pkg of 500,000 cells

producent / nazwa handlowa

Cell Applications, Inc

tryb wzrostu

Adherent

kariotyp

2n = 46

morfologia

smooth muscle

metody

cell culture | mammalian: suitable

powiązane choroby

cardiovascular diseases

Warunki transportu

dry ice

temp. przechowywania

−196°C

Opis ogólny

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

HPASMC has been used to show that IL-22 promotes the growth of pulmonary vascular SMCs via a signaling mechanism that involves NADPH oxidase-dependent oxidation (Bansal, 2013). Inducers of pulmonary hypertension, characterized by thickened pulmonary arterial walls, activate expression of anti-apoptotic Bcl-xL gene via binding of GATA-4 to its promoter; the activation can be suppressed by targeting gata4 gene transcription (Suzuki, 2007). Serotonin induces growth of Pulmonary Artery Smooth Muscle Cells and is able to transactivate the BMP receptor in pulmonary artery SMCs, leading to activation of Smads 1/5/8 via Rho and Rho kinase pathway (Liu, 2009). Pulmonary Artery Smooth Muscle Cells growth can be suppressed by trans-retinoic acid by inducing expression of GADD45A, a known cell growth suppressor, downstream of the retinoid acid receptors RARalpha, RARbeta, RARgamma, RXRalpha, and RXRbeta, indicating possible involvement of retinoic acid in pulmonary vascular remodeling (Preston, 2005). Treatment of Pulmonary Artery Smooth Muscle Cells with plasma containing reduced levels of all-trans RA and 13-cis RA promoted cell growth (Day, 2009). Retinoic acid was also shown to inhibit migration of pulmonary smooth muscle cells by inhibiting PI3K/Akt-dependent reorganization of actin cytoskeleton (Day, 2006). Pulmonary Artery Smooth Muscle Cells were also used to study effects of iron chelation on vascular remodeling and its implications for development of pulmonary hypertension as a result of ROS activity (Wong, 2012). Finally, these cells were used to show that antitumor drugs can selectively target remodeled pulmonary vessels, but not normal vessels (Ibragim, 2014).

Characterization: positive for smooth muscle cell specific alpha-actin expression.

Pochodzenie linii komórkowej

Artery

Zastosowanie

cell growth, signaling, receptor transactivation and suppression, vascular remodeling

Komponenty

Basal Medium containing 10% FBS & 10% DMSO

Uwaga dotycząca przygotowania

  • 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 16 doublings

Rutyna subkultury

Please refer to the HPASMC Culture Protocol.

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Protokoły

Technical information for working with human pulmonary artery smooth muscle cells including thawing, subculturing and cryopreservation

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