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EHIFIDNTP-RO

Roche

Expand High Fidelity PCR System, dNTPack

suitable for PCR, High Fidelity PCR, hotstart: no, dNTPs included

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About This Item

Kod UNSPSC:
41106300
NACRES:
NA.28

zastosowanie

sufficient for ≤1,000 reactions (04738276001)
sufficient for ≤200 reactions (04738268001)
sufficient for ≤40 reactions (04738250001)

Poziom jakości

Właściwości

High Fidelity PCR
dNTPs included
hotstart: no

opakowanie

pkg of 2,500 U (04738276001 [10x250 U])
pkg of 100 U (04738250001)
pkg of 500 U (04738268001 [2x250 U])

producent / nazwa handlowa

Roche

metody

PCR: suitable

moc wejściowa

purified DNA

temp. przechowywania

−20°C

Opis ogólny

Expand High Fidelity PCR System is optimized to efficiently amplify DNA fragments up to 5 kb. The Expand High Fidelity PCR System is composed of a special enzyme mix containing thermostable Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity. The fidelity of DNA synthesis with Expand High Fidelity PCR System shows a threefold increase compared to Taq DNA Polymerase. T/A cloning is recommended.

Zastosowanie

The Expand High Fidelity PCR System is an enzyme blend delivering superior results with twofold higher yield and threefold greater fidelity compared to Taq DNA Polymerase. Choose Expand High Fidelity PCR System for the most sensitive PCR results for genomic targets up to 5 kb in length for:
  • PCR
  • RT-PCR
For maximum convenience, select the 2x concentrated ready-to-use High Fidelity Master.

Cechy i korzyści

  • Use one enzyme for all applications. Enjoy high yield, fidelity, and flexibility.
  • Detect PCR products previously undetectable and avoid false negatives.
  • Achieve successful results from small quantities of template DNA.

Opakowanie

1 kit containing 5 components

Jakość

Each lot of Expand High Fidelity is function-tested in PCR using human genomic DNA and specific primers for collagen (1kb amplified product) and the tPA gene (4.8kb amplified product).

Definicja jednostki

Volume Activity: 3.5U/μL
Each dNTPack contains 10mM additive-free sodium salt nucleotides in a ready-to-use mix.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.
The 5′→3′ polymerase activity of Taq DNA Polymerase and the 3′→5′ exonuclease of the proofreading polymerase combine for improved performance and results; 3′→5′ exonuclease proofreading produces a threefold increase in DNA synthesis fidelity compared to Taq DNA polymerase.

Informacje prawne

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Expand is a trademark of Roche
This page may contain text that has been machine translated.

Tylko elementy zestawu

Numer produktu
Opis

  • Expand High Fidelity Enzyme mix

  • Expand High Fidelity Buffer, with 15 mM MgCl2 10x concentrated

  • Expand High Fidelity Buffer, without MgCl2

  • MgCl2 Stock Solution 25 mM

  • PCR Nucleotide Mix

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Aquatic Chronic 3

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

does not flash

Temperatura zapłonu (°C)

does not flash


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Carlo Foresta et al.
PloS one, 6(3), e15036-e15036 (2011-03-17)
Human papillomaviruses (HPVs) are agents of the most common sexually transmitted diseases in females and males. Precise data about the presence, mechanism of infection and clinical significance of HPV in the male reproductive tract and especially in sperm are not
Navisha Dookie et al.
Infection and drug resistance, 7, 223-228 (2014-09-05)
Moxifloxacin (MXF) has been advocated for the treatment of extensively drug-resistant (XDR) tuberculosis despite resistance to older-generation fluoroquinolones. We investigated the relationship between the minimum inhibitory concentration (MIC) of MXF and mutations in the gyrA and gyrB genes in Mycobacterium

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