DGTP-RO

dGTP

PCR Grade, sodium salt

• Synonim(y): 2′-Deoxyguanosine 5′-triphosphate trisodium salt solution, dGTP-Na₃
• Wzór empiryczny (zapis Hilla): C₁₀H₁₃N₅Na₃O₁₃P₃
• Numer CAS: 93919-41-6
• Masa cząsteczkowa: 573.13
• Numer MDL: MFCD00080337
• Kod UNSPSC: 12352302
• Identyfikator substancji w PubChem: 329798919

Właściwości

• Poziom jakości: 100
• Próba: 99% (dNTP)
• Postać: solution
• zastosowanie: sufficient for 125,000 standard reactions (03732703001); sufficient for 31,250 standard reactions (11969030001); sufficient for 6,250 standard reactions (11934538001)
• opakowanie: pkg of 1,250 μL (11969030001 [100 mM]); pkg of 4 × 1,240 μL (03732703001 [4x100 mM]); pkg of 250 μL (11934538001 [100 mM])
• producent / nazwa handlowa: Roche
• stężenie: <0.9% (dNDP); 100 mM
• temp. przechowywania: −20°C
• ciąg SMILES: [Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@H]3C[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OP(O)([O-])=O)O3)C(=O)N1
• InChI: 1S/C10H16N5O13P3.3Na/c11-10-13-8-7(9(17)14-10)12-3-15(8)6-1-4(16)5(26-6)2-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h3-6,16H,1-2H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,11,13,14,17);;;/q;3*+1/p-3/t4-,5+,6+;;;/m0.../s1
• Klucz InChI: IWGGLKOTEOCWQP-BIHLCPNHSA-K

Opis

Opis ogólny

2′-Deoxyguanosine 5′-triphosphate (dGTP) is made of a guanine nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar linked to a chain of three phosphate residues. It is used by the cells for the synthesis of DNA with the help of DNA polymerase.

dGTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Zastosowanie

2′-Deoxyguanosine 5′-triphosphate trisodium salt is utilized for methods such as DNA sequencing, PCR and other DNA polymerase based DNA synthesis and repair techniques.

Jakość

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Postać fizyczna

solution of dGTP in water (pH 8.3).

Uwaga dotycząca przygotowania

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Inne uwagi

For general laboratory use.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): does not flash
• Temperatura zapłonu (°C): does not flash