DNALIG-RO

T4 DNA Ligase

solution, from Escherichia coli(NM 989), suitable for activity assay

• Synonim(y): dna ligase, t4
• Kod UNSPSC: 12352204

Właściwości

• pochodzenie biologiczne: Escherichia coli (NM 989)
• Poziom jakości: 100
• rekombinowane: expressed in E. coli
• opis: recombinant form of the enzyme from T4 phage
• Postać: solution
• aktywność właściwa: ~3000 units/mg protein
• opakowanie: pkg of 100 U (10481220001 [1 U/μl]); pkg of 500 U (10716359001 [1 U/μl]); pkg of 500 U (10799009001 [5 U/μl])
• producent / nazwa handlowa: Roche
• Parametry: 4-22 °C optimum reaction temp.
• metody: activity assay: suitable
• optymalne pH: 7.2-7.8
• temp. przechowywania: −20°C

Opis

Opis ogólny

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3′-hydroxyl- and 5′-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.

Contents
T4 DNA Ligase, supplied with 10x concentrated ligation buffer that includes ATP.

Specyficzność

Heat inactivation: The enzyme can be inactivated by heating (65 °C, 10 minutes).

Zastosowanie

Ligation of sticky- and blunt-ended DNA fragments.

Jakość

Quality

Tested for the absence of deoxyribonucleases and exonucleases, according to the current Quality Control procedures.

Definicja jednostki

One unit of T4 DNA ligase is the amount of enzyme activity that converts 1 nmol ³²P from pyrophosphate into Norit-absorbable material in 20 minutes at +37 °C. One unit corresponds to 0.2 U determined in the exonuclease III resistance assay. Dilution buffer: 50 mM Tris-HCl, 10 mM dithioerythritol, bovine serum albumin, 500 μg/ml, pH 7.6 (+25 °C).

Volume Activity: 1 x 10³ U/ml; 5 x 10³ U/ml

Uwaga dotycząca przygotowania

Activator: Mg²⁺ (10mM), DTT
Working solution: For dilution of the enzyme Roche recommends using a buffer containing the components of the storage buffer: 20 mM Tris-HCl, 60 mM KCl, 1 mM EDTA, 5 mM dithoerythritol, 50% glycerol (v/v), pH 7.5 (4 °C).

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): does not flash
• Temperatura zapłonu (°C): does not flash