11759779001

BM Chemiluminescence ELISA Substrate (AP)

• Kod UNSPSC: 12352204

Właściwości

• zastosowanie: sufficient for 1,000 tests (ELISA); sufficient for 600 tests (SEAP)
• opakowanie: pkg of 150 mL
• producent / nazwa handlowa: Roche
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C

Opis

Specyficzność

The AP assay system is designed for measuring alkaline phosphatase of various sources (e.g. intestine, placenta, milk, bacteria).

Cechy i korzyści

• Sensitive: Approximately 10fg intestinal alkaline phosphatase can be detected
• High dynamic measuring range: Linear range of more than five orders of magnitude
• Constant light emission: The assay produces a long-lasting light emission instead of a short peak kinetics

Komponenty

1. Alkaline Phosphatase Substrate, CSPD
2. Enhancer, Emerald II
3. Assay Buffer

Zasada

CSPD is dephosphorylated by alkaline phosphatase (AP). The resulting instable dioxetane anion decomposes and emits light at a maximum wavelength of 477nm. Chemiluminescence-enhancing reagents improve the quantum yield of the excited state by more than 500 fold.

Uwaga dotycząca przygotowania

Working solution: Preparation of working solution

The substrate reagent is composed of AP-substrate (bottle 1), Enhancer (bottle 2) and Assay Buffer (bottle 3). Prepare substrate reagent freshly before use. The ratio of the components depends on the application. The substrate reagent is stable at least for one day at 2 to 8 °C.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

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