MAC143

Anti-Strep-Tag Antibody, clone C23.21

• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 200
• białko sprzężone: unconjugated
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: C23.21, monoclonal
• masa cząsteczkowa: observed mol wt ~17 kDa
• oczyszczone przez: using protein G
• reaktywność gatunkowa: all
• opakowanie: antibody small pack of 100 μL
• metody: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• izotyp: IgG1κ
• sekwencja epitopowa: Unknown
• numer dostępu UniProt: N/A
• Warunki transportu: 2-8°C
• docelowa modyfikacja potranslacyjna: unmodified

Opis

Opis ogólny

Strep-Tag is a short amino acid-peptide sequence that exhibits specific binding to streptavidin. It is shown to reversibly occupy the same pocket where biotin is normally complexed. Hence, it is highly desirable for applications involving where efficient purification of fusion proteins. Use of this tag allows for easier detection of fusion proteins in various immunochemical assays. Strep-Tag is shown to be resistant to the action of proteases and can also be used in the presence of mild detergents. Clone C23.21 recognizes fusion proteins with Strep-Tag peptide sequence WSHPQFEK with picomolar affinity. It recognizes both the single and the double Strep-Tag sequence.

Specyficzność

Clone C23.21 is a mouse monoclonal antibody that detects Strep-Tagged proteins. It binds to WSHPQFEK sequence with picomolar affinity.

Immunogen

Recombinant ectodomain of glycoprotein E2 of GB virus (GBV-B), containing a double Strep-Tag (two Strep-Tags separated by a linker peptide)

Zastosowanie

Quality Control Testing

Evaluated by Western Blotting with Strep-Tagged VHH 9.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Strep-Tagged VHH9.

Tested Applications

Surface plasmon resonance: A representative lot detected Strep-Tag in Surface plasmon resonance application. (Meola, A., et al. (2015). J Virol. 89(4):2170-81).

Western Blotting Analysis: A representative lot detected Strep-Tag in human brain extract and GFAP in Western Blotting applications. (Li, T., et al. (2017). Immunol Lett. 188:89-95).

Immunohistochemistry Applications: A representative lot detected Strep-Tag in mouse brain tissue sections in Immunohistochemistry applications (Li, T., et al. (2017). Immunol Lett. 188:89-95).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Strep-Tag, clone C23.21, Cat. No. MAC143, is a mouse monoclonal antibody that detects Strep-Tagged proteins and is tested for use in Immunohistochemistry, Surface plasmon resonance, and Western Blotting.

Postać fizyczna

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable