MABT856

Anti-von Willebrand factor (vWF) Antibody, clone IIIE2.34

clone IIIE2.34, from mouse

• Synonim(y): vWF
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43
• klon: IIIE2.34, monoclonal
• application: ICC; WB
• reaktywność gatunkowa: human
• metody: immunocytochemistry: suitable; western blot: suitable

Właściwości

• pochodzenie biologiczne: mouse
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: IIIE2.34, monoclonal
• reaktywność gatunkowa: human
• opakowanie: antibody small pack of 25 μg
• metody: immunocytochemistry: suitable; western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NP_000543
• numer dostępu UniProt: P04275
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... VWF(7450)

Opis

Opis ogólny

von Willebrand factor (UniProt: P04275; also known as vWF) is encoded by the VWF (also known as F8VWF) gene (Gene ID:7450) in human. vWF is a multimeric plasma glycoprotein that is synthesized by endothelial cells and plays an important in the maintenance of hemostasis. It is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off in the mature form, which is then cleaved into von Willebrand antigen 2 (aa 23-763) and vWF (aa 764-2813). vWF is known to promote adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. vWF also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. Mutations in VWF gene are linked to von Willebrand diseases 1-3 that are common hemorrhagic disorders caused by impaired platelet aggregation. Two isoforms of vWF have been described that are produced by alternative splicing.

Specyficzność

Clone IIIE2.34 is a mouse monoclonal antibody that detects human von Willebrand factor. It targets an epitope with in the VWFD3 domain.

Immunogen

Protease fragment of VWF binding to heparin sepharose from human plasma.

Zastosowanie

Anti-von Willebrand factor, clone IIIE2.34, Cat. No. MABT856, is a mouse monoclonal antibody that detects human von Willebrand factor and has been tested for use in Immunocytochemistry and Western blotting.

Research Category
Cell Structure

Western Blotting Analysis: 1 µg/mL from a representative lot detected von Willebrand factor (vWF) in lysates from Human umbilical vein endothelial cells (HUVEC).

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected von Willebrand factor (vWF) in Human umbilical vein endothelial cells (HUVEC).

Jakość

Evaluated by Western Blotting in human platelets cell lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected von Willebrand factor (vWF) in human platelets cell lysates.

Opis wartości docelowych

~270 kDa observed, 309.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Postać fizyczna

Format: Purified

Protein A purified

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Przechowywanie i stabilność

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable