MABT523
Anti-beta-Actin Antibody, clone RM112
clone RM112, from rabbit
Synonim(y):
Actin, cytoplasmic 1, Beta-actin, beta-Actin
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
RM112, monoclonal
reaktywność gatunkowa
human
metody
immunocytochemistry: suitable
western blot: suitable
izotyp
IgG
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... ACTB(60)
Opis ogólny
Actin is a 43 kDa highly conserved structural protein. There are three main actin isotypes (alpha, beta and gamma) which show >90% amino-acid (aa) homology between isotypes and >98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. Beta actin is one of the two nonmuscle cytoskeletal actins and is relatively stable and appears to remain at normal levels regardless of experimental treatment. It is generally used as an internal control for experiments.
Immunogen
A peptide corresponding to the N-terminus of beta-Actin.
Zastosowanie
Immunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected beta-Actin in A431 cells.
This Anti-beta-Actin Antibody, clone RM112 is validated for use in Western Blotting, Immunocytochemistry for the detection of beta-Actin.
Jakość
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected beta-Actin in A431 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected beta-Actin in A431 cell lysate.
Opis wartości docelowych
~45 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Postać fizyczna
Format: Purified
Inne uwagi
Concentration: Please refer to lot specific datasheet.
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polecane
Klasa zagrożenia wodnego (WGK)
WGK 2
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