MABT1364

Anti-Spectrin beta-II Antibody, clone 2A7

clone 2A7, from mouse

• Synonim(y): Spectrin beta
• Kod UNSPSC: 12352203
• eCl@ss: 32160702

Właściwości

• pochodzenie biologiczne: mouse
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 2A7, monoclonal
• reaktywność gatunkowa: human, rabbit
• opakowanie: antibody small pack of 25 μg
• metody: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• izotyp: IgG1κ
• numer dostępu UniProt: G1SN11
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... SPTBN1(6711)

Opis

Opis ogólny

Spectrin beta (UniProt: G1SN11) is encoded by the SPTBN1 gene in rabbit. Spectrins belong to the superfamily of proteins called F-actin cross linking proteins that function as scaffolding proteins for protein sorting, cell adhesion, and migration. They are principle components of a cell membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. They are actin binding proteins that serves as a membrane organizer and stabilizer. Spectrin beta II is a multifunctional protein that contains lipid-binding sites within its two calponin-homology domains (aa 82-186 and 201-306) and a pleckstrin homology domain (aa 2224-2334) and triple helical segments. Spectrin beta II is generally associated with the cytoplasmic surface of the membrane by attachment to ankyrin.

Specyficzność

Clone 2A7 is a mouse monoclonal antibody that detects beta-II spectrin.

Immunogen

Purified spectrin from frozen rabbit lens membranes.

Zastosowanie

Anti-Spectrin beta-II , clone 2A7, Cat. No. MABT1364, is a mouse monoclonal antibody that detects Spectrin beta chain and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Spectrin beta-II in Immunoprecipitation applications (Bazellieres, E., et. al. (2012). J Cell Sci. 125(Pt 4):919-31).

Immunocytochemistry Analysis: A representative lot detected Spectrin beta-II in Immunocytochemistry applications (Bazellieres, E., et. al. (2012). J Cell Sci. 125(Pt 4):919-31).

Western Blotting Analysis: A representative lot detected Spectrin beta-II in Western Blotting applications (Bazellieres, E., et. al. (2012). J Cell Sci. 125(Pt 4):919-31).

Research Category
Cell Structure

Jakość

Evaluated by Western Blotting in differentiated Caco2 cell lysates.

Western Blotting Analysis: 2 µg/mL of this antibody detected Spectrin beta-II in differentiated Caco2 cell lysates.

Opis wartości docelowych

~275 kDa observed; 271.27 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Postać fizyczna

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable