MABS277
Anti-monoglycylated Tubulin Antibody, clone TAP 952
clone TAP 952, from mouse
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
TAP 952, monoclonal
reaktywność gatunkowa
human, sheep, sea urchin, porcine, mouse, Drosophila, snail
metody
dot blot: suitable
immunofluorescence: suitable
western blot: suitable
izotyp
IgG1κ
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... TUBA1A(7846)
Opis ogólny
Several post-translational modifications of tubulin have been identified in eukaryotic cells. Glycylation is a poly-modification which occurs as lateral branching of glycine chains of variable length identified in axonemal tubulin of Paramecium cilia. This modification has been detected on tubulin and/or cilia/flagella of many unicellular and pluricellular organisms. The differential distribution of distinct polyglycylated tubulin isoforms between cytoplasmic and axonemal compartments in Paramecium indicates that the polyglycylation level of tubulin is highly regulated at the cell level. In Paramecium, the TAP 952 antibody is reactive on cytoplasmic and axonemal tubulin. In metazoan cells and/or cell lines, it is specific for tubulin of motile cilia and primary cilia, and thus useful for motile cilia and primary cilia detection.
Specyficzność
This antibody recognizes the C-terminus of monoglycylated alpha and beta-tubulins.
Can be used as a marker for motile cilia.
Can be used as a marker for motile cilia.
Immunogen
Electroeluted Paramecium axonemal tubulin
Epitope: Amino acids 427-449 of monoglycylated alpha-tubulin and 427-442 of monoglycylated beta-tubulin (Bré et al., 1998, Mol. Biol. Cell 9, 2655-2665)
Zastosowanie
Detect Tubulin using this mouse monoclonal antibody, Anti-monoglycylated Tubulin Antibody, clone TAP 952 validated for use in western blotting, Immunofluorescence & Dot Blot.
Immunofluorescence Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in mouse spermatozoa (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).
Dot Blot Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in synthetic monoglycylated tubulin peptides (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).
Western Blot Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in a panel of total protein extracts from select species (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).
Immunoflourescence Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in lemur, human, and sea urchin spermatozoa (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).
Dot Blot Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in synthetic monoglycylated tubulin peptides (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).
Western Blot Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in a panel of total protein extracts from select species (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).
Immunoflourescence Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in lemur, human, and sea urchin spermatozoa (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
Jakość
Evaluated by Western Blotting on Paramecium total cytoskeletal extract.
Western Blotting Analysis: A 1:50,000 dilution of this antibody detected monoglycylated Tubulin in 10 µg of Paramecium total cytoskeletal proteins.
Western Blotting Analysis: A 1:50,000 dilution of this antibody detected monoglycylated Tubulin in 10 µg of Paramecium total cytoskeletal proteins.
Opis wartości docelowych
~50 kDa observed
Postać fizyczna
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
K Million et al.
Journal of cell science, 112 ( Pt 23), 4357-4366 (1999-11-24)
Tubulins are the major proteins within centriolar and axonemal structures. In all cell types studied so far, numerous alpha- and beta-tubulin isoforms are generated both by expression of a multigenic family and various post-translational modifications. We have developed a primary
Polyglycylation of tubulin: a posttranslational modification in axonemal microtubules.
Redeker, V, et al.
Science (New York, N.Y.), 266, 1688-1691 (1994)
Krzysztof Rogowski et al.
Cell, 137(6), 1076-1087 (2009-06-16)
Polyglycylation is a posttranslational modification that generates glycine side chains on proteins. Here we identify a family of evolutionarily conserved glycine ligases that modify tubulin using different enzymatic mechanisms. In mammals, two distinct enzyme types catalyze the initiation and elongation
A M Callen et al.
Biology of the cell, 81(2), 95-119 (1994-01-01)
Ciliates are very good models for studying post-translationally generated tubulin heterogeneity because they exhibit highly differentiated microtubular networks in combination with reduced genetic diversity. We have approached the analysis of tubulin heterogeneity in Paramecium through extensive isolation and characterization of
Dorota Wloga et al.
Developmental cell, 16(6), 867-876 (2009-06-18)
In most ciliated cell types, tubulin is modified by glycylation, a posttranslational modification of unknown function. We show that the TTLL3 proteins act as tubulin glycine ligases with chain-initiating activity. In Tetrahymena, deletion of TTLL3 shortened axonemes and increased their
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