MABN852

Anti-Amyloid Precursor Protein (APP) Antibody

mouse monoclonal, 2B3

• Synonim(y): Amyloid-beta A4 protein, ABPP, APPI, Alzheimer disease amyloid protein, Amyloid precursor protein, Amyloid-beta precursor protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43
• klon: 2B3, monoclonal
• application: ELISA; ICC; WB; inhibition assay
• reaktywność gatunkowa: mouse, human
• metody: ELISA: suitable; immunocytochemistry: suitable; inhibition assay: suitable; western blot: suitable

Właściwości

• Nazwa produktu: Anti-APP Antibody, clone 2B3, clone 2B3, from mouse
• pochodzenie biologiczne: mouse
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 2B3, monoclonal
• reaktywność gatunkowa: mouse, human
• reaktywność gatunkowa (przewidywana na podstawie homologii): rat (based on 100% sequence homology)
• opakowanie: antibody small pack of 25 μg
• metody: ELISA: suitable; immunocytochemistry: suitable; inhibition assay: suitable; western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NP_000475
• numer dostępu UniProt: P05067
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... APP(351)

Opis

Opis ogólny

Amyloid-beta A4 protein (UniProt: P05067; also known as ABPP, APPI, APP, Alzheimer disease amyloid protein, Amyloid precursor protein, Amyloid-beta precursor protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID: 351) in human. APP undergoes extensive post-translational modification including glycosylation, phosphorylation, and tyrosine sulfation, as well as many types of proteolytic processing to generate peptide fragments. APP is proteolytically processed under normal cellular conditions by alpha-secretase or beta-secretase to generate and release soluble APP peptides, S-APP-alpha and S-APP-beta, and the retention of corresponding membrane-anchored C-terminal fragments, C80, C83 and C99. Subsequent processing of C80 and C83 by gamma-secretase yields P3 peptides. In Alzheimer s disease processing of C99 generates amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42) that form amyloid plaques. Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. They bind transient metals such as copper, zinc and iron. APP can also be cleaved by caspases during neuronal apoptosis. Cleavage at Asp-739 by either caspase-6, -8 or -9 results in the production of the neurotoxic C31 peptide and the increased production of beta-amyloid peptides. In addition to its obvious role in Alzheimer′s disease, the most-substantiated role for APP is in synaptic formation and repair. Its expression is upregulated during neuronal differentiation and after neural injury.

Specyficzność

Clone 2B3 specifically detects Amyloid-beta A4 protein in human and murine neurons. It targets an epitope within 15 amino acids including the beta-secretase cleavage site.

Immunogen

A linear peptide corresponding to 15 amino acids from the C-terminal region of human Amyloid-beta A4 protein.

Epitope: extracellular domain

Zastosowanie

Anti-APP, clone 2B3, Cat. No. MABN852, is a highly specific mouse monoclonal antibody that targets Amyloid beta A4 protein and has been tested for use in ELISA, Immunocytochemistry, Inhibiton Assay, and Western Blotting.

ELISA Analysis: A representative lot detected APP in ELISA applications (Thomas, R.S., et. al. (2011). FEBS J. 278(1):167-78; Thomas, R.S., et. al. (2013). Neuroreport. 24(18):1058-61).

Inhibition Analysis: A representative lot inhibited beta amyloid production in MOG-G-UVW culture media (Thomas, R.S., et. al. (2011). FEBS J. 278(1):167-78).

Western Blotting Analysis: A representative lot detected APP in Western Blotting applications (Thomas, R.S., et. al. (2011). FEBS J. 278(1):167-78).

Immunocytochemistry Analysis: A representative lot detected APP in Immunocytochemistry applications (Thomas, R.S., et. al. (2013). Neuroreport. 24(18):1058-61).

Research Category
Neuroscience

Jakość

Evaluated by Immunocytochemistry in mouse cortical neurons.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected APP in mouse cortical neurons.

Opis wartości docelowych

86.94 kDa calculated.

Postać fizyczna

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Przechowywanie i stabilność

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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