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Key Documents

MABN380

Sigma-Aldrich

Anti-APP-C99 Antibody, clone mC99(70–80)

ascites fluid, clone mC99(70-80), from mouse

Synonim(y):

Amyloid beta A4 protein, ABPP, APPI, APP, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

ascites fluid

rodzaj przeciwciała

primary antibodies

klon

mC99(70-80), monoclonal

reaktywność gatunkowa

human, mouse

metody

immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... APP(351)

Opis ogólny

Amyloid Precursor Protein (APP) is expressed in the brain, kidney, heart, and spleen of fetal tissues; it is induced during neuronal differentiation. The most-substantiated role for APP is in synaptic formation and repair; its expression is upregulated during neuronal differentiation and after neural injury. In adult brain, highest expression of APP is found in the frontal lobe of the cortex. Moderate expression is observed in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Defects in APP are a cause of autosomal dominant Alzheimer′s disease (AD). UP on intracerebroventricular injection of this antibody, A-beta levels significantly decreased in the brain of APPPS1 mouse model of AD. A related antibody, Cat. No. MABN381, Anti-APP-C99, clone mC99(1-7) was raised against a slightly different region of the same APP-C99 protein.

Immunogen

Recombinant protein corresponding to Human APP-C99.

Zastosowanie

Anti-APP-C99 Antibody, clone mC99(70–80) is a highly specific mouse monoclonal antibody, that targets APP & has been tested in western blotting, IP, IHC & IF.
Immunoprecipitation Analysis: A representative lot immunoprecipitated APP-C99 in HEK APPSwe cell lysate (Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).

Immunohistochemistry Analysis: A representative lot detected APP-C99 in APPS1 mice and in human Alzheimer brain tissues
(Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).

Immunofluoresence Analysis: A representative lot detected APP-C99 in HEK APPSwe cells (Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

Jakość

Evaluated by Western Blotting in DAPT treated HEK293 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected APP-C99 in 10 µg of DAPT treated HEK293 cell lysate.

Opis wartości docelowych

~95 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Postać fizyczna

Mouse monoclonal IgGκ ascites
Unpurified

Przechowywanie i stabilność

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

nwg

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

You-Chang Oh et al.
Nutrients, 12(7) (2020-07-11)
Banhasasim-tang (BHS) is an herbal medicine that has been widely used in East Asia to treat various symptoms associated with upper abdomen swelling. BHS has not been studied previously for neuroinflammation or cognitive disorder. Here, we use a lipopolysaccharide (LPS)
Qian Guo et al.
Acta pharmaceutica Sinica. B, 9(3), 590-603 (2019-06-14)
Gene therapy represents a promising treatment for the Alzheimer׳s disease (AD). However, gene delivery specific to brain lesions through systemic administration remains big challenge. In our previous work, we have developed an siRNA nanocomplex able to be specifically delivered to
Selective neutralization of APP-C99 with monoclonal antibodies reduces the production of Alzheimer's A? peptides.
Houacine, Jemila, et al.
Neurobiology of Aging, 33, 2704-2714 (2012)
Elisabetta Lauretti et al.
Annals of clinical and translational neurology, 4(8), 564-574 (2017-08-16)
Consumption of extra virgin olive oil (EVOO), a major component of the Mediterranean diet, has been associated with reduced incidence of Alzheimer's disease (AD). However, the mechanisms involved in this protective action remain to be fully elucidated. Herein, we investigated

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