MABN1724

Anti-GABA(A) Receptor α2 Antibody, clone N399/19

clone N399/19, from mouse

• Synonim(y): Gamma-aminobutyric acid receptor subunit alpha-2, GABA(A) receptor subunit alpha-2, Gamma-aminobutyric acid receptor A2 subunit
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: N399/19, monoclonal
• reaktywność gatunkowa: rat, human, mouse
• metody: immunohistochemistry: suitable; western blot: suitable
• izotyp: IgG1κ
• numer dostępu UniProt: P23576
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... GABRA2 (2555)

Opis

Opis ogólny

Gamma-aminobutyric acid receptor subunit alpha-2 (UniProt P23576; also known as GABA(A) receptor subunit alpha-2, Gamma-aminobutyric acid receptor A2 subunit) is encoded by the Gabra2 (also known as Gabra-2) gene (Gene ID 289606) in rat species. The GABAA receptor (GABAAR) is an ionotropic receptor and ligand-gated ion channel. Its endogenous ligand is the inhibitory neurotransmitter γ-aminobutyric acid (GABA) in the central nervous system (CNS). Upon activation, the GABAA receptor selectively conducts chloride ion through its pore, resulting in hyperpolarization of the neuron. The receptor is a pentameric complex composed of two alpha, two beta, and one gamma subunits arranged around a central pore. Each subunit contains four transmembrane domains with both the N- and C-terminus located extracellularly. In human and rat, there exist six types of alpha subunits (encoded by GABRA1-6), three types of beta subunits (encoded by GABRB1-3), and three types of gamma subunits (encoded by GABRG1-3). The alpha 2 subunit is intially produced with a signal peptide sequence (aa 1-28), which is then cleaved off to yield the mature subunit (aa 29-451) with a large N-terminal extracellular domain (a.a. 29-251), four transmembrane segments (a.a. 252-273, 279-300, 313-334, 420-441) and a large intracellular/cytoplasmic loop (a.a. 335-419) between the third and fourth transmembrane domain.

Specyficzność

Clone N399/19 detects the GABA(A) receptor subunit alpha-2 band only in brain membrane extracts from wild-type rats and mice, but not from Gabra2-knockout mice. Clone N399/19 does not cross-react with GABA(A) receptor subunit alpha-1.

Immunogen

Epitope: Cytoplasmic domain.

Recombinant rat GABA(A) receptor α2 cytoplasmic fragment.

Zastosowanie

Detect GABA(A) Receptor α2 using this Anti-GABA(A) Receptor α2 Antibody, clone N399/19 validated for use in Western Blotting, Immunohistochemistry.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected GABA(A) receptor subunit alpha-2 in 10 µg rat brain tissue lysate.
Western Blotting Analysis: A representative lot detected the GABA(A) receptor subunit alpha-2 target band only in brain membrane extracts from wild-type rats and mice, but not from Gabra2-knockout mice.
Immunohistochemisry Analysis: A representative lot detected GABA(A) receptor subunit alpha-2 immunoreactive regions, includinghippocampus, in rat whole brain sections (Courtesy of Professor. James S. Trimmer, UC Davis, CA, USA).

Jakość

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected GABA(A) receptor subunit alpha-2 in 10 µg human brain tissue lysate.

Opis wartości docelowych

~57 kDa observed. Target band appears larger than the calculated molecular weight of 47.94 kDa due to glycosylation.

Postać fizyczna

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable