MABF2816

Anti-Cortical stromal cells Antibody, clone ER-TR4

• Synonim(y): cTEC
• Kod UNSPSC: 12352203

Właściwości

• pochodzenie biologiczne: rat
• Poziom jakości: 200
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: ER-TR4, monoclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: mouse
• opakowanie: antibody small pack of 100 μg
• metody: immunofluorescence: suitable; immunohistochemistry: suitable
• izotyp: IgMκ
• sekwencja epitopowa: Unknown
• numer dostępu Protein ID: N/A
• numer dostępu UniProt: N/A
• temp. przechowywania: 2-8°C
• docelowa modyfikacja potranslacyjna: unmodified

Opis

Opis ogólny

The thymus is considered as the primary lymphoid organ that is responsible for the generation and maturation of T cells. Thymic epithelial cells (TECs) account for the majority of thymic stromal components. These cells can be divided into cortical and medullary thymic epithelial cells based on their localization within the thymus. TECs have dual germ layer origins, with cortical cells arising from ectoderm and medullary cells arising from the endoderm. In an embryonic thymus, cortical cells are the dominant population, while medullary cells account for most of the TECs in the adult thymus. Cortical thymic epithelial cells are essential for the positive selection of T cells, whereas the medullary thymic epithelial cells (mTECS) play a role in inducing negative selection of highly reactive T cells that are required for establishing central self-tolerance. mTECs participate in self-tolerance by eliminating self-reactive T cells. Cortical cells that support positive selection also support post-selection maturation of single-positive thymocytes. They provide cytokines, such as IL-7 and SCF, to promote early thymic progenitors (ETP) cells and Delta-like ligand 4 (DLL4)-mediated Notch signaling to induce the differentiation of ETPs towards the T lineage. DLL4 is reported to be expressed at high levels in cortical cells, but not in medullary cells. Clone ER-TR4 reacts with epithelial-reticular cells in the cortex of murine thymus. (Ref.: Wang, HX., et al. (2020). Front. Immunol. 10; 3099; Van Vliet, E., et al. (1984). Eur. J. Immunol. 14(6); 524-529; Cordier, AC., et al. (1980). Am. J Anat. 157(3); 227-263).

Specyficzność

Clone ER-TR4 is a rat monoclonal antibody that detects cortical stromal cells.

Immunogen

Thymic stromal cells isolated from C3H mice.

Zastosowanie

Quality Control Testing

Evaluated by Immunohistochemistry in mouse thymus tissue sections.

Immunohistochemistry Applications: A 1:50 dilution of this antibody detected Cortical stromal cells in mouse thymus tissue sections.

Tested applications

Immunofluorescence Analysis: A representative lot detected Cortical stromal cells in Immunofluorescence applications (Tanaka, Y., et al., (2007). Blood. 110(1):107-15).

Immunohistochemistry Applications: A representative lot detected Cortical stromal cells in Immunohistochemistry applications (Van Vliet, E., et al., (1984). Eur J Immunol. 14(6):524-9; Scheijen, B., et al., (2004). J Biol Chem. 279(11):10476-83; Dooley, J., et al., (2006). J Immunol. 176(11):6484-90; Tanaka, Y., et al., (2007). Blood. 110(1):107-15; Moretti, F.A., et al., (2018). Elife. 7:e35816; Hollander, G.A., et al. (1995). Nature. 373(6512):350-3).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Postać fizyczna

Purified rat monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Rekonstytucja

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Przechowywanie i stabilność

Recommended storage: +2°C to +8°C.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1