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MABF260

Sigma-Aldrich

Anti-NLRC5 Antibody, clone 3H8

clone 3H8, from rat, purified by using protein G

Synonim(y):

Protein NLRC5, Caterpiller protein 16.1, CLR16.1, Nucleotide-binding oligomerization domain protein 27, Nucleotide-binding oligomerization domain protein 4

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

rat

Poziom jakości

forma przeciwciała

purified antibody

rodzaj przeciwciała

primary antibodies

klon

3H8, monoclonal

oczyszczone przez

using protein G

reaktywność gatunkowa

human

metody

flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

izotyp

IgG1, kappa

numer dostępu UniProt

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... NLRC5(84166)

Powiązane kategorie

Opis ogólny

The protein named NLRC5, or also known as Nucleotide-binding oligomerization domain protein 27, Caterpiller protein 16.1 (CLR16.1), or Nucleotide-binding oligomerization domain protein 4 and encoded by the gene NLRC5/NOD27/NOD4, plays a role in the homeostatic control of innate immunity and anti-viral defense mechanisms. NLRC5 also likely regulates the NF kappa B and type 1 interferon signaling response pathways induced upon infection. NLRC5 interacts with CHUK and IKBKB and prevents CHUK and IKBKB phosphorylation thus inhibiting their kinase activity. NLRC5 interacts with DDX58 and IFIH1 proteins and blocks the interaction of MAVS to DDX58 proteins. NLRC5 is localized to the cytoplasm. NLRC5 is expressed in spleen, thymus, lung, brain, tonsil, heart and prostate at moderate levels, but NLRC5 expression is dramatically induced by interferons during pathological infections. Traditional immune responses studies have demonstrated that NLRC5 is a specific and master regulator of major histocompatibility complex (MHC) class I genes as well as related genes involved in MHC class I antigen presentation. The expression of MHC class I genes is regulated by NLRC5 in coordination with the RFX components through an enhanceosome-dependent manner, and the involvement of NLRC5 in MHC class I mediated CD8+ T cell activation, proliferation and cytotoxicity has proved to be critical for host defense against intracellular bacterial infections.

Specyficzność

Target specificity of clone 3H8 was confirmed by Western blotting using lysates from cells overexpressing exogenously transfected NLRC5 as well as cells knocked down of endogenous NLRC5 by shRNA transfection (Neerincx, A., et al. (2010). J. Biol. Chem. 285(34):26223-26232). Clone 3H8 epitope is present in human NLRC5 spliced isoforms 1 and 4, but absent from isoforms 2, 3, 5, and 6 reported by UniProt (Q86WI3).

Immunogen

BSA-conjugated linear peptide corresponding to the C-terminal sequence of human NLRC5.

Zastosowanie

Detect NLRC5 using this rat monoclonal antibody, Anti-NLRC5 Antibody, clone 3H8 validated for use in western blotting, IP & Flow Cytometry.
Flow Cytometry Analysis: 0.25 µg from a representative lot detected NLRC5 in Jurkat and Raji cells.

Immunoprecipitation Analysis: A representative lot detected poly(I:C) (Cat. No. 528906) treatment-induced upregulation of endogenous NLRC5 in HeLa cells by IP-Western blotting analysis of both cytosolic and nuclear fractions. A strong NLRC5 nuclear accumulation was seen upon nuclear export inhibition by LepB (Cat. No. 431050) treatment, whereas NLRC5 was mainly cytoplasmic in untreated cells (Neerincx, A., et al. (2012). J. Immunol. 188(10):4940-4950).

Western Blotting Analysis: A representative lot detected NLRC5 in THP-1 cell lysate as well as exogenously expressed FLAG-tagged NLRC5 in lysate from transfected HEK293T cells. Target band detection was greatly diminished using lysates from NLRC5 siRNA-transfected THP-1 cells. (Neerincx, A., et al. (2010). J. Biol. Chem. 285(34):26223-26232).
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Jakość

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected NLRC5 in 10 µg of Raji cell lysate.

Opis wartości docelowych

~205 kDa observed. 204.6 kDa (isoform 1) and 201.3 kDa (isoform 4) calculated. Uncharacterized band(s) may appear in some lysates.

Postać fizyczna

Protein G Purified
Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Andreas Neerincx et al.
The Journal of biological chemistry, 285(34), 26223-26232 (2010-06-12)
Proteins of the nucleotide-binding domain, leucine-rich repeat (NLR)-containing family recently gained attention as important components of the innate immune system. Although over 20 of these proteins are present in humans, only a few members including the cytosolic pattern recognition receptors
Andreas Neerincx et al.
Journal of immunology (Baltimore, Md. : 1950), 188(10), 4940-4950 (2012-04-12)
Nucleotide-binding domain and leucine-rich repeat (NLR) proteins play important roles in innate immune responses as pattern-recognition receptors. Although most NLR proteins act in cell autonomous immune pathways, some do not function as classical pattern-recognition receptors. One such NLR protein is
Priya Ranjan et al.
European journal of immunology, 45(3), 758-772 (2014-11-19)
The NLR protein, NLRC5 is an important regulator of MHC class I gene expression, however, the role of NLRC5 in other innate immune responses is less well defined. In the present study, we report that NLRC5 binds RIG-I and that

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